Sabrina Foerstner Email & Phone Number

Idar-Oberstein, Rp, De

Idar-Oberstein, Rhineland-Palatinate, Germany

Idar-Oberstein, Rhineland-Palatinate, Germany

Edmonton, Alberta, Kanada

PhD Project:Investigated the role of cancer stem cells and oncoproteins in the development of chemoresistance in lymphomaMaster Thesis:Established a novel cellular model and used gene expression array of tumor tissues to study the biology of Post Transplant Lymphoproliferative Disorder (PTLD) (grade 1.0)Other Projects:-Identified molecular mutations in low grade endometrial carcinoma tissue samples to refine risk identification and stratification of recurrent and… Show more PhD Project:Investigated the role of cancer stem cells and oncoproteins in the development of chemoresistance in lymphomaMaster Thesis:Established a novel cellular model and used gene expression array of tumor tissues to study the biology of Post Transplant Lymphoproliferative Disorder (PTLD) (grade 1.0)Other Projects:-Identified molecular mutations in low grade endometrial carcinoma tissue samples to refine risk identification and stratification of recurrent and metastatic cancer patientsTechniques:-Western blot-Conventional and three-dimensional (3D) cell culture-Flow cytometry and cell sorting-Immunohistochemistry-Lentivirus transduction-Transfection-Cell viability assays using trypan blue staining-Colony formation assay-Nuclear/cytoplasmic fractionation-(Quantitative Real-Time) Polymerase chain reaction-Gel Electrophoresis-Reverse transcription-Isolation of nucleic acids from cells and tumor tissue-Fluorescence imaging and light microscopySoftwares:-Graph Pad Prism (for data analysis)-FloJo (for flow cytometry analysis)-Image Studio (for western blot analysis)-ImageJ/Fiji (for image analysis)-nSolver Analysis (for nanostring analysis)Additional Tasks:-Independent literature research, planning and performance of experiments-Establishment and optimization of laboratory protocols-Lab maintenance and general laboratory work (i.e. ordering of reagents) -Restructuring of laboratory inventory (i.e. freezer, benches, antibodies, cell storage)-Detailed documentation and presentation of results during weekly laboratory meetings-Teaching and supervision of an intern student Show less

Tübingen, Department Of Internal Medicine Ii, Laboratory For Dendritic Cells

Analysed the function and differentiation of dendritic cells in patients suffering from multiple myeloma, which play an essential role in the generation of efficient anti-tumor immune responses.Techniques:-Preparation of cell samples from human blood and bone marrow-Cell culture engineering (primary cells and MS-5 cells)-Magnetic cell separation ( MACS) and sorting for CD34+ progenitor cells (FACS) -Cell staining for FACS AnalysisOther tasks:-Independent… Show more Analysed the function and differentiation of dendritic cells in patients suffering from multiple myeloma, which play an essential role in the generation of efficient anti-tumor immune responses.Techniques:-Preparation of cell samples from human blood and bone marrow-Cell culture engineering (primary cells and MS-5 cells)-Magnetic cell separation ( MACS) and sorting for CD34+ progenitor cells (FACS) -Cell staining for FACS AnalysisOther tasks:-Independent learning of software applications-Regular contact with a software expert for problem solving-Presentation of an introductory seminar of the software to the working group-Preparation of an implementation protocolInstruments:-Spectral Flow Cytometer (CYTEK Aurora)Softwares:-FlowJo-FCS Express Flow 7-Graph Pad PrismInternship grade: 1.0Reference Letter available Show less

Tübingen, Interfaculty Institute Of Cell Biology

Determining the effect of Mat2a knock down on proliferation of hepatoma liver tumor cells. Techniques:-cell culture engineering with liver tumor cells.-transient transfection with siRNA-mRNA-Isolation-Reverse transcription-Gel Electrophoresis-Butanol-ether treatment-qPCR for quantification of mRNA-MTT assayInternship grade: 1.0

Ulm University, Institute Of Microbiology And Biotechnology

My tasks as an internship lab support were:-PBMC-isolation-miRNA-isolation and cDNA-synthesis-RT-qPCR for quantificaton of miRNAs-Western Blot-Cell Culture Engineering (with HeLa, Jurkat, Hep3b, HEK, THP-1 and T98G cells)Reference Letter available

Ulm University, Institute Of Microbiology And Biotechnology

Establishment and Optimization of a qPCR method for relative quantification of hsa-miR-124-5p in GM- and M-CSF differentiated human macrophages (grade 1,2).Task: cDNA of mature miRNA from GM- and M-MΦ and the synthetic synthesized hsa-miR-124-5p were used. Via gradient-PCR suitable PCR components and settings regarding primer pair and annealing temperature were tested followed by analysis of specifity of the PCR products using agarose gel electrophoresis. In addition, two SYBR® Green… Show more Establishment and Optimization of a qPCR method for relative quantification of hsa-miR-124-5p in GM- and M-CSF differentiated human macrophages (grade 1,2).Task: cDNA of mature miRNA from GM- and M-MΦ and the synthetic synthesized hsa-miR-124-5p were used. Via gradient-PCR suitable PCR components and settings regarding primer pair and annealing temperature were tested followed by analysis of specifity of the PCR products using agarose gel electrophoresis. In addition, two SYBR® Green mastermixes as well as different reference (mi)RNAs and different concentrations of DMSO were tested by qPCRs. qPCR specifity, efficiency and sensitivity was determined using Ct-value, standardcurve and melting curve analysis. -Isolation of human PBMCs - Differentiation of human monocytes to GM- and M-MΦ- miRNA Isolation - reverse transcribtion of miRNA to cDNA - PCR - Agarose gelelectrophoresis - Quantitative PCR for quantificiation of miRNAsUsed programmes: - Primer Designer5 Version 5.11 - miRprimer- Bio-Rad CFX Manager Version 3.1 - Tecan i-control 1.10 Show less

Ulm University, Institute Of Microbiology And Biotechnology

Validation of high-throughput miRNA-screening in PMA-differentiated THP-1 macrophages by flow cytometry.Tasks: -cell culture engineering with THP-1 cell line- PMA-differentiation of THP-1 monocytes to macrophages-transient transfection of macrophages with siRNA -flowcytometry (gating-strategy regarding macrophage population, viability, transfection and phagocytosis)Used programms:- MACSQunatify Software Version 2.10 - GraphPad Prism Software Version… Show more Validation of high-throughput miRNA-screening in PMA-differentiated THP-1 macrophages by flow cytometry.Tasks: -cell culture engineering with THP-1 cell line- PMA-differentiation of THP-1 monocytes to macrophages-transient transfection of macrophages with siRNA -flowcytometry (gating-strategy regarding macrophage population, viability, transfection and phagocytosis)Used programms:- MACSQunatify Software Version 2.10 - GraphPad Prism Software Version 7.00 Show less

Master Of Science - Ms, Molecular Cell Biology And Immunology, 1,1

Activities and Societies: btS e.V. Compulsory Modules: -Principles of Molecular Cell Biology -Molecular Immunology -Advanced Molecular.

Bachelor Of Science - Bs, Pharmaceutical Biotechnology, 1,6

Activities and Societies: btS e.V. -Mathematics -Physics* -Pharmaceutical Biotechnology -Process Engineering* -General, Inorganic.

General University Entrance Qualification, Food Science, 1,8

Activities and Societies: Member of school council