Cell Culture of adherent, semi adherent, and suspension cell lines (VERO, BHK, MRC5, MDCK, MDBK, CHO, SF9),- Freezing Cells, Thawing Frozen Cells, Cell seeding,- Familiar work in cleanroom class C,- Cell Bank Preparation,- Familiar with cell culture contaminants,- Cell Bank Characterization (DNA & RNA Stability, Copy number), - Analysis of gene sequence, DNA Barcoding,- Writing standard operating procedure (SOP), Documentation

- DNA, RNA and plasmid Extraction from viruses, bacteria, and cells- Synthesis of cDNA- Real-time PCR : . Quantitative Determination of biological contamination . Validation kits . Plasmid copy number by qPCR . Gene Expression Analysis- PCR : . General PCR . HOT Start PCR . Colony PCR . Gradient PCR- Gel electrophoresis, Recovery of DNA from agarose gel- Restriction digestion and ligation- Gene Cloning, Preparation of Competent cells- Flow cytometry : . Analysis of apoptosis . Cell counter . Transduction achievement by GFP- Familiar with principles of GLP and GMP- Principles of Validation- Familiar with standards IS0 9001:2015- Primer designing and analyzing tools