I am continuing my scientific work from my Ph.D. on developing these molecules further. Looking forward to #collaborations and #funding opportunities in this field.

Drug Screening for the Dynamin super-family of proteinsWorked on a drug development project, where I am looking for molecule(s) that would modulate the activity of my target protein. This change in activity would further downstream regulate cellular pathways in membrane trafficking. I first established a kinase assay in the presence of lipids that could be taken to high-throughput screening (HTS). Validated the outcomes of HTS in different assay setups.Two of the validated… Show more Drug Screening for the Dynamin super-family of proteinsWorked on a drug development project, where I am looking for molecule(s) that would modulate the activity of my target protein. This change in activity would further downstream regulate cellular pathways in membrane trafficking. I first established a kinase assay in the presence of lipids that could be taken to high-throughput screening (HTS). Validated the outcomes of HTS in different assay setups.Two of the validated hits showed a specific cellular response in cell-based assays, the rest compounds showed non-specific cellular effects and were eliminated from further studies. These are the first reported inhibitors against my target protein. The potential therapeutic importance of the two compounds could be in the treatment of obesity and breast cancer. In my initial first two years of my Ph.D., I was working on different projects.One of them was, Structural organization of Septin scaffoldsIn this study, we showed that Septin1 was necessary for the structural and functional integrity of Golgi apparatus. Show less

Mechanism of chaperone function of prostaglandin synthase (L-PGDS) for amyloid beta-peptides (Aβ)Solved the crystal structure of L-PGDS (PDB - 5WY9)Optimized the expression and purification of Aβ40 peptide. Did thioflavin assay to test chaperonin activity against amyloidogenic peptides. Biomolecular interaction was measured using surface plasmon resonance and Isothermal titration calorimetry.Electron microscopy (TEM) to see amyloid fibril formation and their inhibition… Show more Mechanism of chaperone function of prostaglandin synthase (L-PGDS) for amyloid beta-peptides (Aβ)Solved the crystal structure of L-PGDS (PDB - 5WY9)Optimized the expression and purification of Aβ40 peptide. Did thioflavin assay to test chaperonin activity against amyloidogenic peptides. Biomolecular interaction was measured using surface plasmon resonance and Isothermal titration calorimetry.Electron microscopy (TEM) to see amyloid fibril formation and their inhibition in presence of L-PGDSSet up crystallization trials of L-PGDS with Aβ40 and ligands (Heme and Retinoic acid)NMR on Bruker Avance II 600 MHz spectrometer to see protein-RNA interaction Show less

Structural and Functional studies of PRDM family of proteins (lysine methytransferases).In this project we cloned, expressed and purified PRDM members 9,14 and 15. Set up crystallization trials of the protein in its apo-form and along with its substrate.Studied binding property of Cellular nucleic acid binding protein (CNBP) with RNA and ssDNA bybiochemical assays.Methods: Protein expression, protein purification, protein crystallization, electrophoretic mobilty shift… Show more Structural and Functional studies of PRDM family of proteins (lysine methytransferases).In this project we cloned, expressed and purified PRDM members 9,14 and 15. Set up crystallization trials of the protein in its apo-form and along with its substrate.Studied binding property of Cellular nucleic acid binding protein (CNBP) with RNA and ssDNA bybiochemical assays.Methods: Protein expression, protein purification, protein crystallization, electrophoretic mobilty shift assay. Show less

Purification and crystallization of histo aspartic protease (HAP) and to determine high resolution crystal structure from Plasmodium Falciparum.In this project I extensively optimized the protocol of expression and purification of HAP protein.Set up crystallization trials manually (hanging drop).

Role of posttranslational modification of tubulin in CancerCell culture (Hela, RPE1 and U2OS cell lines).RNA extraction from cell followed by PCR analysis.Protein extraction from cell followed by immunoblot analysis as well as immunofluorescence analysis