Samantha Fowler Email and Phone Number

Coordination of clinical research sample tracking and movement, data collection and data analysis.

• Analyzed immuno-modulatory effects of lead molecule, BPM31510o Contributed to key experiments resulting in two AACR abstracts in 2017 and 2018 elucidating the role BPM31510 plays in regulating STAT3 activation• Developed reporter assays to demonstrate target engagemento Dissection of NFκB and IFN signaling pathways utilizing commercially available reporter cell lines. • Developed of cellular modelso Validated a dendritic cell model using monocytes isolated from PBMCs and THP-1 monocytes• Developed flow panel for analysis of dendritic cell maturation surface markers• Utilized MSD multi-plex platform to develop cytokine secretion panelo Pioneered and validated 3D cell culture models to demonstrate effect of BPM31510 on spheroid growth• Developed viability assays using the Celigo• Developed immune cell invasion model• Analyzed the effect asset delivery on glucose metabolism and insulin resistance. o Validated target delivery in skeletal muscle cells • Examined glucose uptake in cells after asset delivery in various media conditions• Analyzed murine tissue samples under various diabetic diets for changes in targets and delivery of proposed treatment molecules. o Examined tissue for asset delivery and distribution• ELISA for measure of delivery• Western blot to validate delivery measure and examine changes in metabolic pathways• qPCR for further examination of changes in metabolic markers• Analyzed multiple formulations of proposed therapeutic to ensure success of delivery and increased glucose uptake/metabolic changes in human skeletal muscle cells.o Validated modified assets for specific tissue delivery (skeletal muscle)• Cellular assays for potency of delivery• Far-western analysis for tissue preference of proposed molecule modifiers

• Determined the effects on morphologic maturation and differentiation / functional activation of over expression of the nuclear envelope proteins. o Lamin A (LMNA) and SUN 2 inserted into plasmids and transduced into novel hematopoietic cell lines.• Plasmid preps, restriction enzyme digests, • Developed optimized protocol for transducing two myeloid models. o ER-HOXb8-GMSCF and ER-HOXb8-SCF cells,. • This technique was then used to transduce small inhibitory RNAs against key transcription factors, including GABPα.• Investigation into the possible effects of Liver X Receptor (LXR) functions on the growth and differentiation of a myeloid model were determined using LXR agonists and antagonists. o These data were critical to our studies of the Lamin B receptor in mediating cholesterol biosynthesis (as published in Subramanian et al, 2012, see below).

Zebrafish were tested for the effects of specific drugs on their cardiovascular/pulmonary systems, using high-speed video microscopy and analysis of atrial and ventricular heart function.