Student Researcher Assistant
Costa Rica
• Research assistant at Health Research Unit on a project focused on gene silencing of Sorcin protein in T lymphocytes. • Developed a bachelor thesis focused on the optimization of methods to determine the transfection efficiency of murine T lymphocytes transfected with LGA-PEI nanoparticles. In this I optimized a DNA extraction protocol for transfected T lymphocytes. Also optimized transfection detection by direct uptake of GFP by flow cytometry and amplification of the transfected plasmid… Show more • Research assistant at Health Research Unit on a project focused on gene silencing of Sorcin protein in T lymphocytes. • Developed a bachelor thesis focused on the optimization of methods to determine the transfection efficiency of murine T lymphocytes transfected with LGA-PEI nanoparticles. In this I optimized a DNA extraction protocol for transfected T lymphocytes. Also optimized transfection detection by direct uptake of GFP by flow cytometry and amplification of the transfected plasmid by qPCR.• Experience working with techniques such as murine spleens extractions, selection and culture of T lymphocytes, DNA extraction, E. coli transformation, plasmid maxiprep and qPCR to determine relative expression. • Provided assistance in transfection assays with nanoparticles, real-time proliferation and viability determination (Anexxin and DAPI). • Experience working in the maintenance of an AAALAC certified biotherium for the reproduction and breeding of B6 mice. Show less