Samir Sayed Email & Phone Number

Research Associate II - Translational Research Core Laboratory (TCL)Work on glucokinase hyperinsulinism (GK-HI) included biochemical and PK characterization of patients with unknown glucokinase mutations in order to determine a reliable method for future diagnosis of this ailment (please see list of publications and presentations).Biochemical assay design, development, validation and implementation (Meso Scale Discovery electrochemiluminescence (ECL) platform (SI6000) and general ELISA assay spectrophotometric platforms (using Molecular Devices M5 and M5e models). Design and development of assays include antibody pair selection, antibody conjugation, assay sensitivity improvement, mitigating drug and heterophillic antibody interference, and validation for use with pediatric samples of limited volume). Work closely and foster relationships with Principal Investigator groups, Federal Agencies (NIH, etc.) and institutional departments as part of responsibilities heading the biochemistry core lab of the clinical translational laboratory at the Children’s Hospital of Philadelphia. Keep the lab in compliance with federal standards for College of American Pathologists (CAP) certification required for diagnostic testing.Research Associate II - Translational Research Core Laboratory (TCL)January 2018 - PresentResearch Associate I - Clinical Translational Research Center (CTRC)September 2012 - December 2017Senior Research Assistant - General Clinical Research Center (GCRC)October 2007 – September 2012Research Assistant - General Clinical Research Center (GCRC)March 2004 – October 2007

School Of Medicine

Determined the effect of ligands (from drugs to common chemicals) on the storage, release and absorption of Ca++ in mineralizing cells and tissues. Elucidate receptor-ligand relationships as well receptor specificity (through structural determination, F.R.E.T, etc.) to give more insight into the Endoplasmic Reticulum (ER) in relation to its gates for and ability to store Ca++.

Department Of Chemistry

Instructed the following courses:Introduction to Chemistry (freshman level course)Analytical Chemistry (junior and senior/master’s level courses)X-ray crystallography and Inorganic Analysis (senior/master’s level course)Taught techniques of and data output analysis from various analytical chemistry platforms including:- Infrared spectroscopy- NMR spectroscopy- UV spectroscopy and Fluorometry- Atomic Absorption and Emission- Gas Chromatography- Gel Permeation Chromatography- High Performance liquid chromatography (HPLC) and- Mass Spectroscopy (MS)

Determined major asthma-causing allergen in cockroach FRASS by protein purification (column and spin column extraction), 1D and 2D SDS-PAGE, gelatin zymography, isoelectric focusing (IEF), Western blot analysis and immunostaining.

Halifax, Canada Area

Studied the role of butrylcholinesterase (BuChE) in hallmarks of Alzheimer’s disease (AD), i.e. neuretic plaques (NP) and neurofibrillary tangles (NFT).

Education record

Graduate Work in Engineering and Nanotechnology (Nanometer Scale Science and Engineering)

Bachelor Of Science (B.Sc.), Chemistry

Graduated Summa Cum Laude

Bachelor Of Science (B.Sc.), Biochemistry

Graduated Summa Cum Laude