Sampriti De Email & Phone Number

Atlanta, Ga, Us

Performed analytical formulation screening to detemine components most compatible with active agents. Measured stability and potency of active agents using sandwich ELISA. Performed TCID50 to determine titers and stabilty of Measles and Rubella microneedle patches. Worked as both operator and circulator for production of clinical trial materials in clean room. Trained in GMP practices for record keeping and performing experiments.

Atlanta, Georgia, United States

Assisted with the processing of samples from nOPV and mOPV (poliovirus vaccine) clinical trials that are ongoing in an international effort to eradicate polio. Assisted with the generation of stool suspensions and extraction of RNA using KingFisher Flex.

Basler Lab, Institute For Biomedical Sciences

Optimized several highthroughput cell-based assays using vesicular stomatitis virus (VSV). Titered VSV using plaque assay. Tested for presence of EBOV and MARV proteins of mouse sera and antibody samples from collaborator's lab using Western Blot. Performed mycoplasma detection assays using EnVision Plate Reader. Transfected HEK293T cells using Lipofectamine 2000. Used Lipofectamine RNAiMAX to knockdown genes in HEK293T and A549 cells. Used EBOV minigenome highthroughput screening system to assess activity and cell cytotoxicity of multiple compounds. Optimized VSV antiviral assay to screen activity and cell cytotoxicity of compounds. Quantified fluorescence units of VSV-GFP and Influenza A/PR8-GFP using EnVision and BioTek Cytation 5. Visualized VSV-GFP and PR8-GFP-infected cells using EVoS Digital Microscope. Isolated VSV and Zika-infected RNA samples using TRIzol and generated cDNA using SuperScript III First-Strand Synthesis System for using qrt-PCR to detect levels of viral synthesis. Measured antiviral activity of compounds using ZIKV MR-766. Measured OD of bacterial strains. Performed resazurin microtiter assay for determining drug resistance in M. smegmatis. Experienced in basic mouse handling techniques such as anesthesizing mice using isoflurane and performing euthanasia by cardiac puncture, administering CO2 and cervical dislocation. Utilized mouse models for isolating bone-marrow derived macrophages and measuring antibody levels by intraperitoneal injection of VSV-GP and VSV-GFP. Used GraphPad Prism and Microsoft Excel to plot data from experiments. Trained in BSL-3 TB techniques. Responsible for ordering of lab and office supplies, maintaining laboratory biosafety and compliance, maintaining appropriate tank and water levels of equipment, shipment and delivery of supplies, client/supplier communication, ensuring equipment and personnel are compliant with regulations for opening of new laboratory.

Villinger Lab, Pathology

Produced large quantities of 8C7, 4E11, 7D3 and 36D5 monoclonal antibodies by hybridoma cell culture. Produced milligrams of IgV, Mucin, TIM-3, PDL-1 and IL-21 by S2 cell culture. Maintained adherent Vero and HeLa cell lines. Isolated and amplified CD89 gene from HeLa cells. Isolated, amplified and transformed bacteria to express TIGIT and LAIR1 from Rhesus macaque samples. Tested lots of FBS (fetal bovine serum) to determine which enabled maximum cell viability. Performed various molecular biology techniques, such as: PCR, dot blot, SDS-PAGE, DNA ligation, A-tailing, S2 transfection, blue-white screening, phenol-chloroform extraction, spectrophotometry, E. coli growth and induction with IPTG, JM109 and BL21 bacterial transformation, nucleic acid purification and quantification by Nanodrop. Responsible for budgeting, purchasing of lab supplies, client and supplier communication. Shadowed lab members performing endotoxin tests. Performed general lab duties such as organizing, sterilizing glassware and disposing of biohazard waste. Maintained equipment and created inventory records using Microsoft Excel.

Designed the production of a unique deletion plasmid for Fusarium verticilliodes. Generated primers for unique gene and confirmed accuracy using PCR and gel electrophoresis. Generated mutant strains using fungal transformation with deleted gene of interest. Assisted with plant extraction and other various lab duties.

Weitzstein Lab, Plant Sciences

Performed quality control assays to determine differences between pH and sugar content of pomegranates stored in controlled atmospheres.

Striepen Lab, Center For Tropical And Emerging Diseases

Monitored and maintained human fibroblast cell culture. Conducted PCR, gel electrophoresis, and restriction digests. Shadowed members of the lab working with Toxoplasma gondii. Organized and cleaned work areas and sterilized glassware and other solutions.

Villinger Lab, Yerkes National Primate Research Center

Processed skin biopsies of chimpanzees. Monitored and prepared chimpanzee fibroblast cell culture. Enumerated cells using hemocytometer. Determined cell viability by trypan blue staining. Prepared fibroblast media and other solutions. Purified DNA and rNA and performed PCR and gel electrophoresis. Shadowed lab members performing phycol overlay, EDTA blood processing. Created and updated inventories using Microsoft Excel.

Atlanta, Ga

Acquired proficiency and analyzed blood plasma samples using several FDA-approved HIV Rapid Tests. Prepared dried blood spot (DBS) samples from Ethiopia for HIV screening. Shadowed members of other branches including HIV Incidence team. Worked with team to compile quality control research on topics of HIV Rapid Testing, Malaria Diagnostic Tests, and Tuberculosis Smear Microscopy Reagents.

Master’S Degree, Biology

Bachelor Of Science (Bs), Biological Sciences