Samrat Thouta Email & Phone Number

Surrey, Bc, Ca

Canada

Burnaby, British Columbia, Canada

Vancouver, Canada Area

Lab: Professor Terry Snutch, Michael Smith Laboratories and Djavad Mowafaghian Centre for Brain HealthProject: Investigation of the underlying mechanism of Sudden Unexpected Death in Epilepsy (SUDEP)Techniques: Brain slice patch-clamp electrophysiology in combination with optogenetics to study the neuronal excitability of brain circuits related to SUDEP in validated animal models.

British Columbia, Canada

- Assist in preparation and/or editing of operating grant applications, including data collection and analysis, text preparation and figure generation.- Prepare and edit manuscripts and figures for publication in peer-reviewed journals.- Design, implement and/or supervise experiments studying the electrical properties of proteins involved in heart rate regulation and sudden cardiac death.- Supervise and mentor graduate and undergraduate students in the laboratory setting.

British Columbia, Canada

-My PhD research was focused on understanding the voltage dependent potassium (Kv) ion channel function and how dysfunction is related to disease.-Investigated the structural and molecular determinants of unusual gating properties of voltage-gated potassium channel- human ether-a-go-go-related gene (hERG), which plays a crucial role in cardiac excitability. -Characterized do novo novel KCNQ5 (neuronal channel) mutations that were discovered in children affected by intellectual disability with or without seizures.-Expressed hERG and KCNQ5 in Xenopus laevis oocytes and used electrophysiological techniques including two-electrode voltage clamp, voltage clamp fluorometry, and cut-open voltage clamp towards functional characterization of hERG and KCNQ5 channels.-Performed biochemical analysis such as western blot, protein expression and purification of hERG channels to demonstrate protein-protein interactions. -Investigated key determinants of drug binding in hERG channels.-Experience in using Clampfit 10.3 (Axon Instruments), SigmaPlot11 (Systat Software), or IGOR Pro (Wavemetrics) software to analyse the experimental data.

Spring 2012 - BPK 110 - Nutrition (Teaching Assistant)Spring 2014 - BPK 212 - Food and Society (Tutor Marker)Spring 2015 - BPK 305 - Human Physiology I (Teaching Assistant)

Leicester, United Kingdom

-Four months of intensive taught lab work, involved in understanding of the cellular and molecular processes and techniques in cancer research.-A six-month, independent laboratory research project – investigated structure and function of cytoplasmic regulatory domains of hERG using HiTrap columns with AKTA chromatography systems include affinity, ion exchange, and gel filtration.-Experienced in understanding ion channel trafficking using traditional biochemical techniques such as SDS-PAGE and Western blot analysis. -Created a library of over 50 mutant constructs using combination of molecular biology techniques such as site-directed mutagenesis, Polymerase chain reaction (PCR) etc. -Vector designing using advanced software. -Culturing and cryopreservation of Chinese Hamster Ovary (CHO) mammalian cells.

Hyderabad Area, India

Characterized Bradykinin receptor function by generating stable cell lines and radio ligand binding analysis using saturation binding studies.

Ph.D, Molecular Cardiac Electrophysiology

Activities and Societies: Graduate Student Society (GSS)

M.Sc, Cancer Cell And Molecular Biology

B-Tech, Biotechnology