Samuel Spradlin Email & Phone Number

Philadelphia, PA, US

Engineered new cell lines to reduce manufacturing costs for AAV viral vectorsBuilt plasmid constructs through in-fusion PCR, restriction enzyme digestion, blunt end and sticky end ligation Amplified and purified plasmids from antibiotic-selected bacterial clonesTransfected and maintained mammalian cell linesQuantified vector production by running western.

Philadelphia, Pennsylvania, United States

Presented findings to Principal Investigator and Staff Research Scientists in weekly laboratory meetings Utilized genetic knockouts to quantify changes in protein transcription and expression in retinal tissues of mice to elucidate lipid metabolism pathwaysCarried out western blotting, qPCR, ELISAs and lipid assays to compare transcription and expression.

New Castle, Delaware

Achieved $200k in monthly sales in designated territoryManaged Rotavapor sales and technical inquires for 17 states; assembled customized Rotavapor systems based on throughput needs and solvents used in customer applicationScheduled meetings to discuss laboratory needs with Principal Investigators in academic and industrial settings; generated quotations.

Greater Chicago Area

Managed diagnostic testing for more than 3,600 samples per day, ensuring efficient scheduling and division of labor to complete tests in a timely and accurate manner and within company best practices. Monitored clinical data system to keep delay messages to a minimum, maintaining strict adherence to company documentation practices and industry regulations..

Master Of Science In Engineering - Mse, Bioengineering

Bachelor Of Science - Bs, Biochemistry

Activities and Societies: Molecular Genetics Research Team--- Responsible for the expression and purification of truncated MCM-8 (1-368).