Sam Brook Email & Phone Number

Fairfield, Ct, Us

New Haven, Ct, Us

In-House Screening and Discovery: Orchestrate end-to-end development of a comprehensive small molecule screening funnel, directing in vitro assays, ADME/PK profiling, and in vivo efficacy testing to advance compounds through SAR and lead optimization.Lab Operations Management: Pioneered the buildout of a BSL2-certified oncology drug discovery lab from scratch, leading to full operational status. Implement advanced cell culture and live-cell imaging, and manage critical equipment/reagent procurement. Oversee daily lab operations, ensuring a high-functioning and compliant lab environment. Manage inventory, procurement, and maintenance of critical lab equipment and supplies. Establish and maintain strong relationships with key vendors and CROs to support seamless lab operations.CRO Management and ADME/PK Studies: Establish and manage strategic CRO partnerships for ADME, PK, toxicology, and translational research, collaborating on species selection and large-animal model determination. Direct CRO-conducted in vitro assays, ADME/PK profiling, and in vivo efficacy studies to rigorously evaluate compound potency and advance candidates.Training and Mentorship: Develop and implement SOPs and training programs to maintain consistent methodology and data quality across research teams. Train research staff on lab protocols and best practices. Mentor junior scientists and direct reports, fostering their professional growth and development.Administrative Oversight: Assume responsibility for animal study administrative oversight, including accurate invoice coding, project meeting attendance, and thorough invoice review to ensure timely, error-free CRO payments. Actively participate in recruiting and hiring efforts to build and develop the research team.Budget Management: Manage a $4 million quarterly R&D budget, aligning spending with strategic priorities and achieving cost savings.

Engineered recombinant fusion proteins that advanced to preclinical trials, contributing to the development of biopharmaceutical product candidates.Instrumental in the development of PhaseBio's proprietary ELP biopolymer technology platform, a recombinant biopolymer that extends the circulating half-life of protein therapeutics. Contributed significantly to optimizing the expression systems used to supply a robust pipeline of fusion proteins for evaluation and development.Spearheaded the production and development of bioengineered peptides through large-scale fermentation using bench-scale bioreactors, ensuring high-quality outputs.Led the downstream purification of initial fusion protein products using advanced AKTA FPLC systems, including affinity, ion exchange, and hydrophobic interaction chromatography (HIC) techniques.Collaborated cross-functionally with GLP lab, process development, and technical operations

New York, Ny, Us

Contributed to the development and establishment of a nanoparticle-based targeted drug delivery system for delivery of small molecule kinase inhibitors the tumor microenvironment.- achieved tumor-specific drug delivery resulting in durable control of tumor growth - enhanced efficacy by combined administration of radiation therapy and anti-tumor kinase inhibitors without adverse off-target effectsIdentificiation and characterization of a novel mechanism of resistance to targeted therapy in PIK3CA mutated cancer.- developed acquired resistance in cultured cell line and patient-derived mouse models to recapitulate the complexities of the tumor microenvironment and allow more accurate predictions responses to therapiesptimized and implemented mechanistically-relevant cell-based immunoassays to evaluate biological activity of novel therapeutic agents and combinations; characterized the transcription factors that regulate genes responsible for resistanceSuccessfully designed and conducted preclinical anticancer drug evaluation studies using the following skills and techniques:Cell-based bioassays performed in-vitro: mammalian cell culture, gene editing using siRNA- and shRNA-mediated gene silencing, DNA recombination and retroviral plasmid construction, cell proliferation and apoptosis assays, immunoprecipitation, Western Blot, isolation & purification of DNA, RNA, and protein from cells and tissues, high-throughput quantification of phenotypic markers using flow cytometry.In-vivo techniques: small animal disease modeling and patient-derived xenograft surgery, administration of IP, IV, IM, and SQ drug injections, oral gavage dosing, monitoring anti-tumor efficacy and measuring tumor volume throughout treatment regimens, in-vivo imaging modalities using fluorescent and bioluminestcent reporters (IVIS Lumina imaging system) to monitor tumor progression and metastasis. Nanoparticle based drug delivery platforms, tumor cell isolation, radiation therapy

New York, Ny, Us

Applied xenograft disease models to explore novel treatment options for patients with high grade glioma Extraction, purification, and culture of tumor stem cells derived from human glioma tissue; characterization of potential therapeutic agents via analysis of gene and protein expression

Cambridge, Massachusetts, Us

Investigated the developmental biology (and sub-type specification) of neocortical projection neurons; experiments aimed to elucidate the molecular genetic controls underlying segmental specificity in corticospinal projection neurons.Performed mechanistically-relevant studies using transgenic mouse models and engineered cell lines; Provided support to the lab through experimental techniques in molecular biology, animal surgery, immunohistochemistry. Worked independently and managed multiple projects simultaneously.Relevant skills & techniques: Primary neuron isolation and cell culture, harvested murine neonatal neuronal tissue, DNA vector construction and cell transfection, recombinant plasmid cloning in E. coli, recombinant protein expression and isolation, large-scale plasmid preps, DNA-mediated transformation, primer design, DNA sequencing and sequence analysis, confocal and immunofluorescence microscopy and imaging, histology, in-situ hybridization, in-vitro transcription, ELISA, tissue homogenization, intracardial perfusion, in-utero electroporation

Us

Studied mast cell function and immune response in a rodent model of asthma by performing preclinical studies of asthma pathophysiology including in vivo drug treatment, bronchoalveolar lavage, tail vein injection, organ dissection to harvest and isolate murine HSCs, culturing these cells for in vitro characterization, targeted differentiation of stem cells into various immune cell types. Provided technical support to lab members investigating the development and maintainenance of airway inflammation

Bachelor'S Degree, Neuroscience

Education record

Graduate School Of Biomedical Science, Biomedical Sciences, General

Neuroscience