Samuel Florence Email & Phone Number

London, England, United Kingdom

London, United Kingdom

• Scientist
• Played a key role in the characterisation, pre-clinical research and Candidate Selection into lead drug candidate efficacy in both cell and primary line based assays, as well as within primary tumours.
• Procurement of primary tissues with resource management of tissue between projects.
• Report writing for patents and pre-IND documents.
• Extensive flow immuno and phenotypic assay design, development and deliverance to SOP standard.
• Working within a matrix project lead environment in a fast paced company to deliver Candidate Selection criteria.

London, United Kingdom

• Process Development Scientist:
• Played a key role in the development and implementation of strategy in bioprocess development, with focus of adapting process to enable scale-up suitable for clinical manufacture.
• Planned and executed small-scale optimization studies to improve research grade process, creating a more GMP compliant process.
• Played an active role in the evaluation and selection of manufacturing equipment needed for the clinical production of cell-based products, including authoring and reviewing process protocols.
• Provided manufacturing input for regulatory filings, particularly around raw materials.
• Assisted in the tech transfer of a key company product to clinical manufacturing organisation.

London Bioscience Innovation Centre

• Associate Scientist role:
• Responsible for maintaining primary cell cultures derived from human tissues.
• Creation of frozen cell stocks, cell pellets and supernatant stocks for later analysis in functional and analytical assays, together with tracking and archiving vial numbers.
• Regularly performed analytical assays aimed at assessing cell phenotype and activity, including but not limited to; Flow-cytometry and Luminex.
• Ensured that all data generated was well recorded and presented in a way which was suitable for uploading to electronic laboratory notebook system.
• Interim laboratory manager (Feb17-Oct17):o Responsible for maintaining laboratory consumable stocks and implementing ordering when necessary to support the research efforts of the team. Generated and maintained the.

Imperial College Incubator

• In addition to research scientist role; Laboratory management and safety officer. Research scientist role
• Regularly performing assays to support the Kesios pre-clinical drug discovery cascade. Assays performed include ELISA, western blotting and qRT-PCR.
• Cell line analysis to confirm the expression of target proteins and interactions by immunoprecipitation and protein arrays using cell and tumour lysates.
• Responsible for maintaining cultures of adherent and non-adherent cell lines, including cancer cell lines.
• Creation of cell banks and cell pellets for later analysis in functional and biochemical assays, together with tracking and archiving vial numbers.
• Solely responsible for ensuring laboratory consumables are adequate and implementing ordering when necessary to support the research efforts of the team. Generated and maintained the company inventory.

Dartford, United Kingdom

• Production of biological products such as antigens through mammalian cell culturing techniques, using both batch and fed-batch systems. Batch scale ups start at 1.8ml, and can reach in excess of 100 litres.
• Carrying out regular aseptic work through the use of microbiological safety cabinets (ISO class 5 environment), as well as through the use of sterile connections. Ensuring strict adherence to production paperwork.
• Sampling of cultures for metabolites (glucose & lactate) in addition to determining culture pH.
• The use and maintenance of liquid nitrogen storage systems and cell banks.
• Management of stock levels through the use of electronic stock maintenance systems.
• Working hard with the rest of the team to adhere to a set manufacturing schedule and elevating any problems to line management.

Dartford, United Kingdom

• Updating of company COSHH reports, with the use of current MSDS reports provided both by the company and through self sourcing.
• Creation of COSHH processes in which hazards for various steps of manufacturing processes are noted in order of use.
• Applying hazard categories and assessing the risks of all chemicals held on site using the Globally Harmonised System of classification (GHS).
• Creation of an assessment index spreadsheet containing details of which chemicals and buffers are used, frequency of use, risk posed by each chemical/buffer, which technicians are using them and finally the locations.
• Interacting with a variety of different departments in order to meet tight deadlines for the completion of the risk assessment project.
• Ensured organisation of accumulated files and folders, allowing ease of access for other EHS staff.

Greenwich

Dartford, United Kingdom

• Updating and creation of COSHH reports using the global harmonised system (GHS) for all buffers and processes used on site.
• Updating and sourcing copies of material safety data sheets (MSDS) for all chemicals used on site.
• Creation of an assessment index spreadsheet containing details of which chemicals and buffers are used, frequency of use, risk posed by each chemical/buffer, which technicians are using them and finally the locations.
• Ensured organisation of accumulated files and folders, allowing ease of access for other Abbott staff.