 Hired, supervised and trained student workers Wrote protocols and Standard Operating Procedures for animal procedures as well as other techniques performed in the lab Assisted the principle investigator with experimental design and data analysis Presented research and data at lab meetings and scientific conferences Maintained inventory and ordered laboratory supplies as needed  Maintained a breeding colony of mice Performed animal techniques such as rodent anesthesia, jugular cut-downs and injections, IP and IM injections, euthanasia, profusion and tissue collection (including bone marrow, microvillus membranes, and micro vessels of the brain) Performed glucose tolerance testing, insulin sensitivity testing and NMR on mice Performed RNA extractions and real-time PCR analysis on tissue samples Performed Western blot analysis Performed ELISA’s, FPLC, cholesterol and triglyceride enzyme assays Performed FLOW cytometry analysis Processed, cut and prepared slides of tissue samples for staining or immunohistochemistry (using a cryostat or microtome)

 Oversaw mandatory regulatory compliance according to Animal Welfare Act Regulations (AWARs), Public Health Service Policy on Humane Care and Use of Laboratory Animals (PHS policy), AAALAC guidelines, as well as institutional policies Investigated all compliance complaints and administered compliance re-training  Coordinated IACUC personnel and facilitated semiannual inspections and audits of all animal use areas at the University Authored, distributed and presented a Compliance Report to the IACUC Committee on a monthly basis Oversaw and evaluated the institution's animal program to ensure they were consistent with the recommendations in the Guide for the Care and Use of Laboratory Animals (the Guide), the AWARs, and PHS Policy.

 Performed aseptic sterile technique for tissue culture and radiolabeled cholesterol efflux assay on human plasma fractions Performed phospholipid and cholesterol enzyme assays Performed Western blot analysis Performed anion exchange chromatography  Learning an apoptosis assay using FLOW analysis Prepared solutions and general laboratory upkeep