Signal Transduction in Cancer and Metabolism / Dr. Aurelio Teleman• Cultivation of mammalian cells (MCF7, HeLa, U2OS, Hek 293T and Jurkat)• Cell transfection for overexpression or knock down of GOI• Dual Luciferase reporter gene assays in mammalian cells to study the effect of gene knockdown, depletion of nutrients or treatment with pharmacological active compounds• CRISPR/Cas9-mediated gene editing in mammalian cells• Generation of stable mammalian cell lines expressing GOI• Expression and purification of recombinant proteins in E.coli• Broad range of molecular biology techniques including: Cloning, different PCR techniques (e.g. site-directed mutagenensis, Gybson Assembly), DNA and RNA extraction from tissue and cultured cells, cDNA synthesis, qPCR, DNA Sequence analysis, Gel ectrophoresis of DNA and RNA, Western Blot, IP• P-element, PhiC31 and CRISP/Cas9 mediated germ line transformations of D. melanogaster• Lab managemant duties

Developmental Biology / Dr. Anne EphrussiMain focus: Drosophila Melanogaster/ Cell polarity and RNA localisation• Responsible for the EMBL Drosophila Transgenic Service - Microinjection in Drosphila Embryos - P-element, PhiC31 and CRISPR/Cas9 mediated transgenesis.• Maintenance of D. melanogaster stock collection• Molecular Biology (PCR, Cloning, Western Blot)• Flourescence In Situ Hybridisation (FISH) and Immunostainings of D. melanogaster Ovaries• Epi-flourescence and Confocal microscopy• Y2H Assays• Protein expression and purification in E.Coli for Crystallization and Structure Analysis• Lab organisation

Developmental Biology / Dr. Stephen Cohen Main focus: Drosophila Melanogaster / Growth and Metabolism• Responsible for the EMBL Drosophila Transgenic Service - Microinjection in Drosophila Embryos - P-element mediated transgenesis• Molecular Biology• Lab organisation

Assay Development and Reagent Production / Babette Aicher, Ph.D.• Development, performance and analysis of biochemical and cell based kinase assays for identification and optimization of pharmacological active compounds• Expression of recombinant proteins in E.coli• Purification of proteins and antibodies

Target Identification / Dr. Günter Brönner• Setup of phenotypic screens based on classic Drosophila genetic• Development of biochemical high-throughput triglyceride analysis for phenotypic characterisation of Drosophila lines

Department of Plant Pathology and Crop Protection Generell Plant Pathology and Plant Protection / Dr. Birger Koopmann• Resistance testing of brassica napus against pathogenic fungi• Analysis of phytoalexins in plant and herbicide residues in soil by HPLCAgricultural Entomologie / Dr. Bernd Ulber• Breeding and care of plants for experiments• Design, processing and analysis of laboratory, greenhouse, and outdoor experiments• Maintenance of insect stocks