Molecular Biology: • Western Blots• Elisa development• RNA extraction and RT PCR• Blood chemistry analysis using clinical analyzer• Luminex assays• Flow cytometry using FACS Calibur and Imagestream (includes new assay development)• Cell sorting using FACS Aria (one color)• Cloning • Cloning of genes of interest from cDNA • Restriction digests, gel purification, and ligations• DNA Mini-preps, Mega-preps and Giga-Preps• Tissue Staining• Sectioning of tissue using Cryostat• Staining of fresh tissue section and paraffin embedded section• Antigen retrievalAnimal Skills• Isolation of various muscles and organs from mice for protein, RNA , or flow cytometry analysis• Denervations • Oral gavage, Sub Q, IP, IM, and Tail Vein injections• Terminal blood collection (Cardiac puncture and Orbital bleed)• Glucose measurements• Blood pressure measurements• IP Glucose tolerance test (70 mice with 5 time points)• PK/ PD studies• Breeding, genotyping and colony maintenance• Adoptive transfer• Implantation of osmotic pumps into mice• Subcutaneous injection of tumor cells into hind leg of nude mice• Isolation of tumors for analysis• Isolation and culture of mMSC cells from bone marrow• T-cell isolation from splenocytes and lymphocytes Tissue Culture/ Cell Based Assays• Maintain and perform experiments using various cell lines (both stable and primary).• Immunoflourescence• Viability assays• Culture of isolated spenocytes for memory cell and T-cell activation experiments• cGMP measurement in T84 cellsData Analysis/ Personal Skills• Proficient in using Excel and Prism for data analysis, graphs, and tables.• Very proficient with Powerpoint and comfortable with presenting data• Work very well in teams or independently• Excellent communication, presentation, and critical thinking skills.