Freelance Editor – Dallas, TX 2016 –2018Regulatory; Pharmaceuticals• Provided freelance editing services to clients in the pharmaceutical industry, specializing in regulatory affairs.• Edited and proofread regulatory documents, including INDs, NDAs, DMFs, labeling, and SOPs, ensuring compliance with regulatory guidelines and requirements.• Verified consistency, clarity, and accuracy of scientific and technical information.• Collaborated with clients to understand their editing needs, project requirements, and deadlines.• Maintained confidentiality and adhere to client confidentiality agreements.• Worked independently and managed multiple projects simultaneously.• Edited scientific manuscripts, grants, and technical reports based in a variety of clinical and biomedical research disciplines.• Ensured the edited documents adhere to the standards and requirements of peer-reviewed journals or funding agencies.Skills Developed- Regulatory Knowledge, ICH guidelines, 21 CFRs, Scientific/ Medical writing, Data interpretation, Collaboration, Attention to details, Document review, GDP, Effective communication.

Scientist; Research, Drug discovery and target identification, Regulatory affairs.• Designed and executed experiments to elucidate disease mechanisms, validate drug targets, and evaluate efficacy of potential therapeutics for Leukemia.• Utilized various techniques, such as molecular biology, cell culture, high-throughput screening, and biochemical assays, to identify candidates for pre-clinical studies.• Analyzed and interpreted experimental data, utilizing statistical analysis and data visualization tools. • Developed methods for new molecules, purification of potential target proteins, and their identification.• Designed and carry out biological assays to validate protein targets.• Deciphered cancer biomarkers with diagnostic and therapeutic potential.• Assisted in the preparation and review of regulatory documents, including INDs, NDAs, and marketing applications, ensuring compliance with regulatory guidelines.• Collaborated with cross-functional teams to gather and review data for regulatory submissions.• Assisted in responding to regulatory queries and facilitated communication with regulatory authorities.• Maintained regulatory databases and document management systems, ensuring proper organization and version control of documents.• Assist in the preparation and management of regulatory inspections and audits.• Stayed up to date with regulatory changes and industry best practices through continuous learning and research.Techniques - High-throughput drug screening in drug-induced cancer cell models using JANUSTM automotive workstation, Cytotoxicity and cell proliferation assays, Affinity chromatography, DARTS, Western blotting, Elisa, animal xenograft models, and various molecular and cell biology techniques

• Identified microRNA-based biomarkers for lung cancer and other thoracic malignancies.• Studied behavior of human cancer cells in culture and assays in response to knockdown, as well as over-expression, of specific microRNA.• Investigated whether miRNA expression levels induced M1/M2a polarization of macrophages.• Determined effect of microRNA-mediated control of macrophages and implications in cancer treatment.

Dissertation Research –A novel interaction between 12-lipoxygenase and integrin subunit β4 plays a role in cancer progression.• Determined structural basis for interaction between β4 integrin subunit and 12-LOX.• Studied how β4 regulates 12-LOX cellular localization and activity.• Identified functional implications with regard to cell survival, cell migration, proliferation, and tumor growth.Techniques: Recombinant DNA technology, cloning, real-time PCR, Northern blotting, mammalian cell transfections, transcription factor promoter analysis systems, cell migration and invasion assays, SDS-PAGE, Western blotting, FACS, immunoprecipitation, ELISA, immunofluorescence, cell culture, DNA and RNA isolation and purification, protein purification and GST pull-down assay, cell proliferation / cell survival / TUNEL assays, HPLC, LC-MS

In Quality Control department• Developed antibodies, proteins, and ELISA kits.• Ran quality control analysis on ELISA kits.

Thesis: Regulation of INO2 Expression in Yeast.Techniques used :Gene cloning, restriction analysis (mapping, digestion, ligation), transformation, Mini, Midi and Maxi plasmid preparation, spectrophotometric estimation of DNA and proteins, PCR, RT-PCR, Real time PCR, construction of recombinant plasmid for cloning purposes, agarose gel electrophoresis, SDS-PAGE, In vitro transcription gel extraction of nucleic acids, DNA purification, immunodiffusion, protein assays, DNA sequencing ,western, northern and southern blotting, screening, isolation and maintenance of pure cultures, Fluorescence Microscopy

Taught Basic Life Mechanisms and Basic life Laboratory Course.Duties- • Taught introductory Biology, Botany and Zoology Lab for under-graduate students.• Prepared lab instrument and lab material. • Help with dissections• Conduct and grade exams and quizzes.

Duties performed as Medical Technologist:• Blood CollectionProficiency in Venipuncture• MicrobiologyCulture, isolate and identify pathogen from pathological samples, collection of pathological samples, Antibiotic sensitivity tests, Monteux Test, Staining- Grams stain, Acid-fast Stain, Albert’s staining, etc• Clinical BiochemistrySugar Analysis (Fasting, Post prandial, Glucose tolerance test), Kidney /Liver/Cardiac function test, Lipid profile, Electrolytes, Minerals, Enzymatic study, etc• Clinical PathologyUrine - Physical, Microscopic and Chemical analysis, Pregnancy testStool - Physical, Microscopic and Chemical analysisSputum - Routine analysis, AFB detection, C.diphtheria detection.• Hematology: Hemoglobin, Hematocrit, Blood indices, RBC, WBC and Platelet counts, ESR, Blood grouping, Bleeding and Clotting time, Prothrombin time.• SerologyWidal test for Typhoid, VDRL test for syphilis, HIV test, RA test, Australian Ag for Hepatitis, ASO and CRP tests.• Blood Banking and ImmunohematologyBlood grouping, Rh factor, Du variant, Anti-globulin test, and Compatibility test, Blood group choices for transfusion.• Automation and InstrumentationHandled Coulter MAXM for Hematology, Blood cell counters (8 parameters), Auto analyzers for biochemistry, Statfax Semi-auto analyzer, Centrifuges, Binoculars high magnification Nikon microscope, Photoelectric calorimeter, Electric water bath, Incubator, Hot air oven, Autoclave, etc.• Special TechniquesRadio immunology (RIA), ELISA, Thyroid function test, Serum drug levels, Hormonal levels, Electrophoresis, ANA antibody, PSA, etc