Sangeetha Stewart Email & Phone Number

Ann Arbor, Michigan, United States

Department: Protein Sciences and Structural Biology> Expertise in protein upstream and downstream processing> Express challenging proteins in various expression system (Bacterial, mammalian, insect cell culturing)>Purification method developments for various targets; expertise in AKTA FPLC systems and chromatographic techniques - IEX, HIC, SEC, Affinity> Characterization of protein targets - enzyme kinetics, plate based assays ; Biophysical Characterization - SPR analysis, TSA/DSF, DLS, SEC, Fluorescence spectroscopy, protein-protein interaction, protein-small molecule> Worked on some cool targets ; Integral membrane proteins, inclusion bodies, hard-to-express proteins, selenocysteine incorporated enzymes, protein complexes

Greater Detroit Area

Department: Vaccine R&D > Worked in pre-clinical vaccine (antigen-based vaccines) development R&D>Worked on vaccine development programs for infectious diseases: Chlamydia, HSV2, RSV, Influenza, MERS, Pertussis, Allergy: Peanut allergens>Upstream processing of glycoproteins/therapeutic antigens in mammalian systems (HEK293F), >Purification of glycoproteins using FPLC- AKTA PURE system, Affinity Chromatography, SEC, HIC >Mammalian cell culture maintenance – Vero, HEp2, PK15, MDCK and HEK293F cells>Analytical Assays: Western Blot, Dot Blot, SDS PAGE, Bradford assay, BCA, A280, Particle Sizing, Stability studies for nanoemulsion based vaccine formulations using analytical techniques• Immunology based assays – ELISA, Plaque and Neutralization assays, virus inactivation studies, working knowledge of ELISPOT, Luminex >Maintaining laboratory inventories, Data analysis and presentation, Writing and reviewing SOPs, general laboratory duties

Greater Nashville Area, Tn

• Working in a collaborative research setting with scientists and research associates in the protein sciences department• Proficient in upstream and downstream processes of target proteins and enzymes• Experience in setting up biochemical assays, protein-protein interaction studies, fluorescence based assays, in vitro characterization of bio molecules, label free analysis of protein-fragment interaction using Biacore systems (Surface Plasmon Resonance) • Proficient in bacterial cell expression system, experience in mammalian systems- HEK293F cells for making eukaryotic proteins, worked on shake flasks and bioreactors• Purification of native and tagged proteins, experience in IMAC, Ion Exchange Chromatography (IEX), Gel Filtration (SEC), HIC columns, experience in using AKTA AVANT systems• Maintained laboratory inventories and data sheets, prepared buffers and stock solution, orders and purchasing, general laboratory duties

Columbus, Ohio Area

• Worked in a collaborative environment with post-docs, research assistants, PhD students, as well as independently• Skilled in performing microbiology and molecular biology techniques according to SOPs like nucleic acid isolation, genotyping, assisted in tissue sectioning FFPE and OCT samples, histology studies-IHCs and H&E staining, western blots• Trained in in vitro cell culture techniques and cell based assays• Maintained transgenic mice colonies, assisted in small animal surgeries and organ harvests •Maintained detailed record of all protocols, conducted literature review, have experience in trouble shooting

Master'S Degree, Chemical And Biomolecular Engineering

Bachelor Of Technology (B.Tech.), Chemical Engineering

Activities and Societies: Indian Institute of Chemical Engineers, Youth Red Cross, Entrepreneurship Development Cell.