Sangeetha Stewart Email & Phone Number

Ann Arbor, Michigan, United States

Department: Protein Sciences and Structural Biology> Expertise in protein upstream and downstream processing> Express challenging proteins in various expression system (Bacterial, mammalian, insect cell culturing)>Purification method developments for various targets; expertise in AKTA FPLC systems and chromatographic techniques - IEX, HIC, SEC, Affinity>.

Greater Detroit Area

• Department: Vaccine R&D > Worked in pre-clinical vaccine (antigen-based vaccines) development R&D>Worked on vaccine development programs for infectious diseases: Chlamydia, HSV2, RSV, Influenza, MERS, Pertussis.
• Immunology based assays – ELISA, Plaque and Neutralization assays, virus inactivation studies, working knowledge of ELISPOT, Luminex >Maintaining laboratory inventories, Data analysis and presentation, Writing and.

Greater Nashville Area, TN

• Working in a collaborative research setting with scientists and research associates in the protein sciences department
• Proficient in upstream and downstream processes of target proteins and enzymes
• Experience in setting up biochemical assays, protein-protein interaction studies, fluorescence based assays, in vitro characterization of bio molecules, label free analysis of protein-fragment interaction using Biacore.
• Proficient in bacterial cell expression system, experience in mammalian systems- HEK293F cells for making eukaryotic proteins, worked on shake flasks and bioreactors
• Purification of native and tagged proteins, experience in IMAC, Ion Exchange Chromatography (IEX), Gel Filtration (SEC), HIC columns, experience in using AKTA AVANT systems
• Maintained laboratory inventories and data sheets, prepared buffers and stock solution, orders and purchasing, general laboratory duties

Columbus, Ohio Area

• Worked in a collaborative environment with post-docs, research assistants, PhD students, as well as independently
• Skilled in performing microbiology and molecular biology techniques according to SOPs like nucleic acid isolation, genotyping, assisted in tissue sectioning FFPE and OCT samples, histology studies-IHCs and H&E.
• Trained in in vitro cell culture techniques and cell based assays
• Maintained transgenic mice colonies, assisted in small animal surgeries and organ harvests
• Maintained detailed record of all protocols, conducted literature review, have experience in trouble shooting

Master'S Degree, Chemical And Biomolecular Engineering

Bachelor Of Technology (B.Tech.), Chemical Engineering

Activities and Societies: Indian Institute of Chemical Engineers, Youth Red Cross, Entrepreneurship Development Cell.