Sanjay B Niranjan Email & Phone Number

Vadodara, Gj, In

Pune, Maharashtra, India

Pune, Maharashtra, India

Senior Manager, Bioassay Development Team, Biotech

Bengaluru Area, India

Building contract research services platform for Biologics and Invitro assay of following invitro areas,Biological assay: Bioassay development and validation for various biosimilars and insulin analogues using Primary and secondary culture (Primary adipocytes, Hepatocytes, Pancreatic Islets & Cardiomyocytes), Insulin Phosphorylation assay, In-cell western, Adipogenesis, lipogenesis & free fat cells assay using Primary adipocytes (BAT). Neutralizing antibody Assay: Assay development, validation and Biosimilars evaluations, neutralizing antibody assay by ELISA or cell based assaysADA Assay: Development and validation of ADA assay, equivalence with reference, demonstrate dilution linearity and parallelism, Detect ADA in presence of high circulating drug concentrations and Drug–ADA complex. Ligand binding assays or Enzyme Linked Immunosorbent Assays (Sandwich, Bridging, Direct/Indirect, Competition ELISA)Ligand binding assay: Radio ligand binding assay & Receptor occupancy assaySafty toxicology: Assay development and validation for safety Toxicology

Pune Area, India

Program leader of drug discovery programs in disease area of immuno oncology, Gastro-intestinal diseases, Inflammation & metabolic disorder and team lead of Protein purification & expression group. Team lead and training to the team, looking for innovative solutions and new technology, implication of new technology, presentation, project management and establishing different assay to pre-clinical evaluations. Validation assay development related to proteins and stable cell lines. Responsible to arrange target meeting, direct communication with collaborators & presentations.Molecular & cell biology: Genetic manipulation, Protein expression and purification, Stable clone generation, clonal selection and identification, cell based assay and primary culture isolation. Biochemical and cell based Assays: Enzyme assays (Inhibition, Activation & Kinetic analyses), ELISA assay development, Protein-protein interaction assays, Ligand binding assays (Competitive binding, Saturation binding & Dissociation Kinetics), metabolic and inflammation based assays, Real time gene expression analysisDrug discovery Program:Team Lead for preclinical drug development which includes Reagent generation, assay development and compound screening and new target proposals and exploration. Client interface client presentation, project management Ex-Vivo assays, Arranging target meeting, direct communication with collaborators & presentationsBioassay for Biosimilars evaluations: - Assay development & validation, and biosimilarity evaluations, proposals and report preparationsTook the primary responsibility of execution of projects mainly of oncology and inflammatory disorder therapeutic areasFinding new drug target, feasibility analysis and make project proposals before senior management of global discovery team, after approval-design and execute required research activities with a small project team, Training and team development and Skill development

Molecular & Cell Biology Division-Advinus Therapeutic Ltd, Pune, India

Program leader of drug discovery program of metabolic and inflammation disease area and also was a Program leader for one of Neglected Diseases. Optimization and Validation of in-vitro assays [cell based, immunological and biochemical] and ex vivo assay, Stable clone generation, clonal selection and identification, molecular biology and cell biology, Si-RNA, cloning, site directing mutagenesis and protein engineering. Isolation and Purification of Proteins and Enzyme kinetics studies from bacteria, Baculovirus & mammalian system. Primary culture isolation (islets, primary hepatocytes & cardiomyocytes) and ex-vivo assay development. Radio-ligand binding assay, Intracellular Ca2+ and cAMP measurement (HTRF based). Published two Research Article in reputed International journals

Molecular & Cell Biology - Metabolics, Advinus Therapeutic Ltd, Pune, India.

Research Project: • Type II Diabetes target, Hypertension, GPCRs, Enzymes based projects – Role in Metabolic diseases.• target for Neglected Diseases Malaria.Responsibilities/ Achievements: 1. Optimization and Validation of in vitro [cell based, immunological and biochemical] and ex vivo assay model system.2. Stable clone generation using Mammalian cell lines. 3. Generation of Different construct and site directed mutagenesis using molecular biology techniques. 4. Mechanism of action studies using molecular biology tool and Protein biochemistry. 5. Developed Protein-Protein Interaction assays.6. Isolation and Purification of Proteins and Enzyme activity studies from bacteria, Baculovirus & mammalian system.7. Isolation and culture of islets (pancreas) from rats for assessment of glucose stimulated insulin secretion. 8. Isolation and culture of Rat Hepatocytes from rats for enzyme activation studies.9. Radioligand binding assay. 10. Intracellular Ca2+ and cAMP measurement (HTRF based) using Flex station from stable clones generated in-house.11. Received CEO Excellence award from Company CEO for significant contribution.

Molecular & Cell Biology - Metabolics, Advinus Therapeutic Ltd, Pune, India.

Research Project: • Type II Diabetes target, Hypertension, GPCRs, Enzymes based projects – Role in Metabolic diseases.• Inflammation drug Target Responsibilities/ Achievements: 1. Optimization and Validation of in vitro [cell based, immunological and biochemical] and ex vivo assay model system.2. Stable clone generation using Mammalian cell lines. 3. Generation of Different construct and site directed mutagenesis using molecular biology techniques. 4. Isolation and Purification of Proteins and Enzyme activity studies from bacteria, Baculovirus & mammalian system.5. Radioligand binding assays. 6. Experience in setting Molecular biology, Cell culture, radioactivity labs.

Prof. D. N. Rao Lab., Department Of Biochemistry, Indian Institute Of Science (I

Research Project: • Methyl Directed DNA mismatch repair in Haemophilus influenzae, • Protein-Protein interaction studies by using Type III Restriction modification system as model system.• Mutagenesis studies of MutH of Haemophilus influenzae.

Srf, Research And Development Management

Doctor Of Philosophy - Phd, Physiology, Obesity, Inflamation, Myokines

Msc In Biotechnology, Biotechnology

Bsc, Life Science