My PhD thesis focused on two important biological questions: 1) understanding how polarised growth is regulated in fission yeast Schizosaccharomyces pombe. To this end, i optimised a physiological live-cell imaging protocol that enabled us to perform long term imaging to study polarised growth.2) Studying nuclear positioning in fission yeast, which is important for precise segregation of genetic material between the daughter cells. To address this, i used live-cell imaging, yeast… Show more My PhD thesis focused on two important biological questions: 1) understanding how polarised growth is regulated in fission yeast Schizosaccharomyces pombe. To this end, i optimised a physiological live-cell imaging protocol that enabled us to perform long term imaging to study polarised growth.2) Studying nuclear positioning in fission yeast, which is important for precise segregation of genetic material between the daughter cells. To address this, i used live-cell imaging, yeast genetics and drug perturbations. Supervisor : Prof Ken SawinFunding: Principals Career Development Fellowship and Edinburgh Global Research Fellowship (University of Edinburgh) and Wellcome Trust (to Prof. Sawin) Show less

Investigating how BAR-domain proteins are involved in regulation of cathrin-dependent and cathrin-independent endocytic pathways using live-cell imaging, Drosophila cell culture and cell biology techniques.