Sankar Rajendran Email & Phone Number

# High throughput methods for assays and ELISA quantification were developed and implemented for various PE industrial enzymes. Characterization of various enzymes based on their kinetic activity, stability, and substrate specificity. # ELISA development for PPS (pentason polysulfate) detection as per FDA guidelines. This product and technology was sold out to US antibody production giant Abnova. (https://www.abnova.com/protocol_pdf/KA2273.pdf)# Development of stabilization buffer for HRP conjugates with validity of 1 year shelf life in 4˚C# ELISA kit development for HCP (Host cell proteins of CHO and E.coli cells) molecules as per FDA guidelines# Automation: Tecan EvoWares Robots & Kbiosystems colony picker- Tecan automation script writing, troubleshooting, AMC, inventory maintenance, validation, and constant interaction with vendors.# Molecular cloning: Primer design, TA cloning, PET vectors, PCB302 binary vector, RNA isolation, CDNA synthesis, and PCR optimization # Protein expression: E. coli, Bacillus sp. and Nicotiana benthamiana# Purification: monoclonal and polyclonal antibody by Protein A/G/L and desalting columns# Conjugation: HRP, biotin and ALP conjugation with Proteins and Antibodies# Localization: Plant viral movement protein and silencing suppressor protein in planta and Brdu assay for bone marrow regeneration in mouse# Drug assays: Lymph node, bone marrow cells from animal models such as BALB/c, C57 mice and also in some cell lines (Insulin CT-3 cell line). Drug administration through (IP, IV &S/C)# Cell lines: Hybridoma cell lines, confluence check, passaging and handling of 96, 24, T25 and T75 flasks

Listed skills include Elisa Validation And Tech Transfer, Elisa Kit Development, Immunoassays, Cell Based Assays, and 39 others.