(Formerly PhenomeX, IsoPlexis, Berkeley Lights)

Antibody Discovery, Immunized Mouse Tissue Processing, PBMCs, Biomarker-based Immune Cell Enrichmentj, Single B Cells Screening for Antibody Secretion, Beacon Optofluidic, Cell-based Assays, Hybridoma, RNA Isolation, RT-PCR, Repertoire and scRNA Sequencing library Prep (Illumina NGS), Cloning, Cell Culture, Transfection, Automation, Characterization (BLI Octet, ELISA).(Reason of separation: massive lay off)

• Antibody and Antigen Constructs Design in mammalian expression vectors to support multiple Antibody Discovery Campaigns for research and early stage cancer immunotherapy drug development, • PCR, Cloning (Gibson/Restriction), mutation, Fc-engineering, Sequencing, SnapGene, Plasmid Prep, • Single B cell isolation from immunized mouse PBMC, RT-PCR, HTP cloning, sequence check in PipeBio,• Cell Culture, Transfection (HEK293/CHO), Protein Purification (Affinity), SDS-PAGE, Endotoxin, • Lentiviral cloning/packaging, transduction, piggyBack Transposon cloning (non-viral), stable cell line,• Prepared SOPs and documented data in Benchling.

Managing daily oligonucleotide synthesis in Dr. Oligo 192 Oligo Synthesizer (Biolytic Lab Performance), oligonucleotide quality control analysis by mass spectrometry in MALDI-8020 (Shimadzu) and normalization in Biomek FX/NX Robotics Liquid Handling Automated Workstations (Beckman Coulter) to ensure consistent high-quality products and excellent on-time delivery. Maintaining records in the CLIMS (Customer Laboratory Information Management System), an online ordering and data management user interface.

Research Scientist III, Tuberculosis Drug Discovery, CGIDR• Driving the identification of novel drug targets in M. tuberculosis and other non-tubercular mycobacteria (NTM) including M. avium and M. abscessus.• High-throughput screening (HTS), functional validation and development of multiple series of small molecule putative drug-candidates based on literature and identified targets. • Performed genetic analysis of the strains after drug treatment by developing species specific primers and probes for the target validation/diagnosis by PCR, qRT-PCR and RNAseq.• Cellular assay development to determine the metabolic changes in mycobacteria (microsomal stability, reporter gene expression and membrane potential disruption assays).• Treatment and testing on several wild type and target mutation/deletion/over-expression strains.• Experiments in the BSL3 and BSL2 facilities.

1. Developed several genetically manipulated bacterial and algal transgenics for the industrial production of high value products like renewable energy (biofuel), advanced biomaterials including spider silk, nanocellulose, leghaemoglobin.2. Developed expertise in primer designing, SnapGene, Blast, Primer3, designing plasmid construct, PCR, cloning (Golden Gate/Gibson/Restriction Enzyme), site-directed mutation, DNA sequencing, bacterial transformation, screening of transgenic phenotypes, maintenance of plasmid database and bacterial stocks.3. Developed expertise in DNA/RNA extraction, RNA-Seq and NGS sample preparation, gene expression analysis by qRT-PCR.4. Efficient in recombinant protein expression and purification in bacterial/cyanobacterial system, SDS-PAGE, ELISA, Western Blot, EMSA. 5. Documented and reported test results regularly in electronic (ELN) and lab notebook, and presented the results to the team meetings.6. Supervised and trained new colleagues, team members and interns.

Designed and performed experiments in cyanobacterial molecular biology and actively participated in the scientific discussion and technology transfer process.

Worked in the R&D, Product and Business Development teams and major duties were Contract Research Activities and Molecular Biology Product Development.Contract Research Activities: Cloning, Mutagenesis, DNA Sequencing, Protein Expression, Gene Expression Analysis.Molecular Biology Product Development: DNA and Protein Ladder, Taq/Pfu DNA Polymerases and PCR Kit, DNA Ligase, DNase, RNase and RNase Inhibitor.

PhD in Biotechnology on 'Regulation of stress response factors and their roles in mycobacteria'. Identified novel drug targets and studied polyphosphate metabolism in Mycobacteria tuberculosis.Techniques: primer designing, PCR, Nucleic Acids isolation and purification, molecular cloning and vector development, gene mutation/insertion/deletion/silencing, microbiology lab practices, transformation and electroporation, protein expression and purification, codon optimization, reporter gene techniques, promoter strength comparison, SDS-PAGE, Western Blot, ELISA, Protein-Protein/DNA interaction studies, EMSA, Blot Overlay, immuno-precipitation, preparation of protein for biophysical techniques, RNA isolation, qRT-PCR.