Sara Manenti

Sara Manenti Email and Phone Number

Analista virus testing @ Eurofins BioPharma Product Testing Italy
Milan, IT
Sara Manenti's Location
Milan, Lombardy, Italy, Italy
About Sara Manenti

Sara Manenti | Molecular & cellular BiologistRecently graduated in Biology applied to biomedical research with a year and a half of hands -on reasearch experience and a BSc in Biological Science (University of Milan).I have always felt attracted to the world of science and research, particularly throughout my academic journey, I have been fascinated by the intricate complexities of cancer and its profound effects on both individuals and society. Over the past year, I've had the opportunity to fully immerse myself in the world of scientific research, experiencing both its positive aspects and its challenges. Regarding myself, I am a compassionate person who always strives to help others and offer support whenever possible. My determination and curiosity drive my approach to problem-solving, as I believe every obstacle presents an opportunity for growth. I am convinced that a comprehensive understanding of a subject can only be achieved through the relentless pursuit of knowledge and insights. I am a glass-half-full kind of person who spreads positivity and energy, always ready to brighten the mood with enthusiasm.I am deeply committed to ongoing professional development and learning, driven by my enthusiasm for advancing scientific understanding

Sara Manenti's Current Company Details
Eurofins BioPharma Product Testing Italy

Eurofins Biopharma Product Testing Italy

View
Analista virus testing
Milan, IT
Website:
eurofins.it/pt
Employees:
30
Sara Manenti Work Experience Details
  • Eurofins Biopharma Product Testing Italy
    Analista Virus Testing
    Eurofins Biopharma Product Testing Italy
    Milan, It
  • Ifom
    Research Fellowship
    Ifom Jan 2024 - Jun 2024
    Milano, Lombardia, Italia
    The main aim of my project is to compare the effect of β-catenin regulation in a different CRC cell line, DLD-1. Both DLD-1 and Caco-2 cell lines have a mutation in Adenomatous Polyposis Coli (APC), a crucial protein involved in regulating the β-catenin pathway, particularly in the Wnt signaling cascade. APC acts as a tumor suppressor by controlling β-catenin levels through its role in the β-catenin destruction complex. My work focuses on testing the effect of β-catenin, E-cadherin, and other important proteins involved in AS regulation via depletion using siRNA transient knockdowns to validate the role of these proteins in this cell line. I also generated mock and β-catenin-depleted DLD-1 cells grown in sparse and polarized conditions for deep RNA-seq analysis to uncover AS events in this cell line.MAIN ACTIVITIES:•Cell culture•Transient knock down with siRNA transfection•Immunofluorescence•Conditioned medium production•Confocal microscopy•RNA extraction•DNA extraction•PCR•RT-PCR•Agarose gel electrophoresis•Protein extraction from cell lines•Western blot
  • Ifom
    Trainee For Master'S Degree
    Ifom Oct 2022 - Dec 2023
    Milano, Lombardia, Italia
    MyosinVI, a crucial motor protein involved in diverse cellular processes such as endocytosis and cell migration, undergoes functional regulation through alternative splicing, giving rise to two distinct isoforms: myoVI long and myoVI short. Previous studies conducted in Simona Polo's Lab (IFOM) have revealed the selective expression of the myoVI short isoform in ovarian tumors, emphasizing its pivotal role in cancer cell migration. Intriguingly, depleting myoVI in cells exclusively expressing the short isoform impairs their migratory capacity, a phenomenon absents in cells expressing all myoVI isoforms. This project aims to definitively ascertain and validate the role of myosin V Ishort in promoting ovarian cancer cell metastasis. To achieve this, we sought to establish isogenic OVCAR-8 cell lines that stably express either the epithelial myoVI long or the oncogenic myoVI short isoforms. MAIN ACTIVITIES:•Cell culture•Electroporaton technique•Plasmid transfection•RMCE technique•Single cell cloning•Basic knowledge of Crispr/cas9 techninque•Conditioned medium production•DNA extraction•PCR•RT-PCR•Agarose gel electrophoresis•Protein extraction from cell lines•Western blot•Immunoprecipation assay
  • Università Degli Studi Di Milano
    Trainee For Bachelor'S Degree
    Università Degli Studi Di Milano Nov 2019 - Dec 2019
    Milano, Lombardia, Italia
    Use of heterologously expressed proteins in Xenopus laevis oocytes for analyzing ionic channels through electrophysiological techniques; studying the functionality of reporter proteins like GUS and GFP under different promoters in Arabidopsis thaliana; overexpressing GUS in Arabidopsis thaliana to conduct biochemical analysis of its catalytic activity.

Sara Manenti Education Details

Frequently Asked Questions about Sara Manenti

What company does Sara Manenti work for?

Sara Manenti works for Eurofins Biopharma Product Testing Italy

What is Sara Manenti's role at the current company?

Sara Manenti's current role is Analista virus testing.

What schools did Sara Manenti attend?

Sara Manenti attended Università Degli Studi Di Milano, Università Degli Studi Di Milano.

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