Sara Pastore Email and Phone Number

• Master's degree in Molecular Biotechnology Thesis: "Personalised Gene Therapy for Patients with Primary Ciliary Dyskinesia (PCD)" Job-related skills: Work with bacteria: transformation, cloning, plating.  Work with different cell lines: immortalised cells (HEK293T, A549, A375, HUH7, J-LAT, SK-MEL), cells' patients.  Agarose gel and electrophoresis  In vitro working: in vitro reaction (digestion)  Using optical microscope  Work under the cabinet in sterility  Data analyze with GraphPad Software®  Work with different CRISPR-Cas systems (Cas9, nCas9, dCas9, Cas13)  Base editing with nCas9 System in vitro (HEK293T) with lipofection and ex-vivo on cells' patients with AAV  Genome extraction  Plasmid DNA extraction  Transfection with plasmids Transduction with lentivirus vector and adeno-associated viral vectors  Reading absorbance, fluorescence and chemioluminescence on plate with Varioskan™ LUX multimode microplate reader  Design of sgRNA  Design oligonucleotide  Ligation and cloning of oligonucleotide  FACS  Digital PCR  PCR  Real-time PCR  Basic bioinformatics  Basic use of RStudio  Use Benchling tool  Work with thermocycler Protein quantification with BSA assay  Western blot and imaging of membrane with ChemiDoc™ Molecular Imager