Graduate Research Student
London, Ontario, Canada
Developed innovative methods to integrate Endothelial colony forming cells (ECFCs) into extracellular matrix (ECM) biomaterial scaffolds, both independently and in co-culture with adipose-derived mesenchymal stromal cells (ASCs), with the goal of enhancing vascular regeneration and wound healingGained proficiency in the isolation and maintenance of primary human cell lines, including ASCs from adipose tissue and ECFCs from umbilical cord bloodPerformed ECM-derived scaffold analysis including Young’s modulus measurements and characterization of ECM components, including sulphate glycosaminoglycan and hydroxyproline contentConducted cell behaviour assessment employing a range of quantitative and qualitative techniques, such as flow cytometry, Live/Dead® and PicoGreen® assays, RT-qPCR, and histologyExperimentation in vivo to evaluate cell retention and host vascular cell infiltration within implanted scaffolds, utilizing in vivo bioluminescence imaging and immunohistochemistryIndependently designed, executed, and optimized experiments, demonstrating meticulous time management and organizationMaintained detailed laboratory notes and adhered to or improved upon established SOPsResearch selected for presentation at conferences up to the international level