Sarah Hugo Email and Phone Number

• Genotyping lead on a wide variety of CRISPR projects; such as investigating new transfection reagents, knock-ins, onboarding difficult to transfect cell types, and validating or troubleshooting current protocols. Collaborated with scientists on experiment design and execution. Produced large high quality data sets quickly and efficiently.• Developed and scaled the Sanger genotyping process used to determine CRISPR mediated knockout from spin tubes to 384 well sample format using bead based purifications and liquid handlers. Independently implemented all controls.• Accelerated onboarding of multiple cell types by developing and executing Transfection Efficiency Assays.• Integral member of the cross functional team that onboarded CRISPR to Recursion's high-throughput phenomics platform.• Collaborated with software engineers and data scientists to develop multiple applications, including customized primer design, picklist generators and analysis tools.• Member of high-throughput whole transcriptomics team using Novaseq 6000.• Performed Western blots to correlate protein loss with phenoprint strength, genomic knockout and gene expression levels.• Key team member on investigating the implementation of CRISPRa/i technology.• Assisted in developing the qPCR process, driving the effort to move from 96 well cell culture plates to 384 well in order to implement automation and better match the phenomics platform.

Lab Specialist in Bray lab, studying the effect of genetic variation on blood clotting, as well as defining MiRNA regulation in megakaryocytes and platelets. Adding phlebotomy, primary cell culture, platelet purification and aggregation, as well as various other lab techniques to my repertoire. Writes and manages IRB and IACUC protocols. Recruits and manages participants in our study.

Performed and directed specialized laboratory techniques and procedures in a research lab using zebrafish as a model for metabolic diseases. Executed experiments to characterize a novel fatty liver mutant (Hugo et al., 2012). Carried out all aspects of a forward genetic screen to identify and characterize new mutants with altered liver metabolism. Assisted students and Post Docs in a variety of projects, often requiring significant modification of standard protocols. Managed safety inspections, inventory, and training of students.Lab Skills• PCR techniques including genotyping, HRMA, qPCR, and colony pcr • Microsatellite mapping• TLC • Lipid analysis • Fluorescent and Light microscopy • Radioactive tracer studies• Cloning • Western Blots • Bench top chemistry, extractions, colorimetric assays• Aseptic technique • Media and standard preparation and standardization• Quick and motivated to learn new techniques and adapt existing protocolsZebrafish specific skills• All skills necessary to create transgenic zebrafish lines using the Tol2 system• Extensive zebrafish husbandry experience • Microinjection• Forward genetics screen • In-Situ Hybridization• Dissections • Blood extraction • microgavage

Prepared and analyzed samples for a wide range of analytes in various types of media in accordance with established methods utilizing numerous wet chemistry and colorimetric techniques. Managed own tasks while collaborating with others to meet overall performance goals. Performed routine maintenance and troubleshot analytical instrumentation. Qualified on over 30 methods. Served as a member of the safety committee.Skills• Bench top chemistry, titrations, distillations, extractions, colorimetric assays• Operating, maintaining, and troubleshooting instruments• Quick and motivated to learn new techniques and adapt existing chemistries• Media and standard preparation and standardization• Quality Control techniques (ex: standard curves, spike recoveries, data trends)