• Defining etiological agents from clinical samples using amplicon-based next-generation sequencing- Analyzed clinical characteristics and epidemiological study, performed whole genome sequencing of viral genome extracted from natural hosts and clinical samples, and conducted phylogeographic analysis.• Validating the SFTS diagnosis by RT-qPCR using amplicon-based next-generation sequencing- Addressed discrepancies between two RT-qPCR diagnoses using amplicon-based MinION sequencing and analyzed the genomic characteristics of SFTSV using phylogenetic and genetic reassortment analyses. • Influenza virus propagation- Conducted Influenza virus propagation, virus quantification, and titer quantification by plaque assay.• Discovery of novel Paramyxoviruses from natural hosts in the Republic of Korea- Conducted nucleic acid extraction, molecular screening of Paramyxoviruses using RT-PCR, phylogenetic analysis of Paramyxoviruses’ partial sequence, and host species identification.• Whole-genome sequencing and genomic characterization of novel Paramyxoviruses harbored by rodents and shrews as a potential zoonotic pathogen in the Republic of Korea- Designed multiplex PCR primers for target enrichment, conducted library preparation, and performed whole genome sequencing using MinION ONT.