Sarvani Mandaleeka Email & Phone Number

California, United States

Foster City, California, United States

Waltham, Massachusetts, United States

⮚ Generate efficient cell lines producing desired monoclonal antibodies by stable transfection of CHO cells using Transposon mRNA⮚ Experiment design and execution to determine recovery (post transfection), Growth productivity at 24 well plate to 24 well deep well plate level⮚ Improve cell culture performance by optimizing transfection and growth conditions like cells, DNA, mRNA ratios, stationary to shaking stage during the selection⮚ Titer Analysis using Octet, purification using Kingfisher Flex protocols⮚ Analysis of purified Monoclonal Antibodies using Size Exclusion Chromatography⮚ Successful Glycan release and N-Glycan profile Analysis using Rapifluor-MS N-glycan kit in different Antibody modalities⮚ Generating reports, consolidating and presenting experimental and Analytical data in Team meetings⮚ Procurement of crucial materials and supporting purchasing activities when necessary

Boston, Massachusetts, United States

⮚ Effective integration of Landing pad sequences in target sites by engineering Jurkat cell lines using CRISPR cas9⮚ Single cell clonal cell line cell line development to generate clones containing successfully integrated Landing pads at target sites after nucleofection using systems Like LONZA nucleofection⮚ Analysis cell culture samples for fluorescent protein expression using iQue3⮚ Single cell and bulk sorting using Sony MA900 FACS sorter and Imaging sorted cells using Cloneselectimager (CSI)⮚ Generation of plasmid templates from glycerol stock inoculations, purification of plasmids⮚ Generation of desired ds DNA by designing primers and amplification by PCR, purification using Minelute kits⮚ Design and execute experiment on Hamilton STAR equipment in collaboration with automation team to automate few Cell line development steps⮚ Generate, analyze, and record high-quality data using Ginkgo LIMS, documentation, and database tools⮚ Presentation of experiment data at cross-functional team meetings

Waltham, Massachusetts, United States

⮚ Effectively generating stable clones with desired titer and quality by single cell cloning using advanced systems like Solentim VIPS and Cell Metric CLD, delivering the clones and reports to complete deliverables within project timelines⮚ Experiment design and execution to determine Growth, productivity, performing Titer and metabolite Analysis in Shake flasks and Bioreactors⮚ Improve cell culture performance using Fed-batch production and parameter optimization studies in High Throughput, Automated systems like Ambr 250 HT and Modular⮚ Developed Octet based ELISA-like method to enhance easy and effective titer measurement in coordination with clients⮚ Report generation, and Data presentation to clients and in Cross-functional team meetings⮚ Generation of Research cell banks using cGLP⮚ Upstream development using bench-scale Applikon bioreactors (2-10L) ⮚ Procurement of crucial materials and supporting purchasing activities for internal and client projects

Cambridge, Massachusetts

- Efficiently transfected CHO cells using transposase system by electroporation enabling the production of pools transiently expressing desired antibodies- Evaluated the performance of transfected pools by analyzing VCD, titer and metabolites using ViCell, NOVA, OCTET and Excel spreadsheets- Single- cell cloning of the desired pools resulting in the generation of high performing clonal population- Assessment of growth, productivity, impact of factors like Anti-clump, product quality and stability of the clones by extensive fed-batch studies- Successful generation of 4 clones that produce over 5 mg/mL of bi-specific antibody by optimization of transfection and Fed- batch conditions

Waltham, Massachusetts

- 24 well deep well trace metal studies to evaluate trace element impact on cell growth and productivity- High throughput screening of cell cultures and Metabolite Analysis using Automated equipment like BioHT and its maintenance- Attained a significant reduction in high mannose (improved product quality) in 2 subclones- Conducted Fed-batch shake flask studies to determine the effect of different culture conditions on CHO subclones; - 2L, 50L Bioreactor and AMBR (Automated mini bioreactor) experiments- Media development/ Amino acid solubility testing; Achieved solubility of 15 Amino acids in Feed- Data Analysis using softwares and MS Excel- Cell culture and cell banking

Cambridge, Ma

- Microengraving using CHO cells; Imaging and Scanning the micro engraving chips using high throughput screening to identify single cells- Analysis of the micro engraving chips to identify high performing single cells using various software like Genepix, Picocell, CellCelector- Conditional selection of desired single cells by cell picking and culturing the cells in 96 well plates- Optimization of protocols to improve results

Boston, Ma

-Synthesis of Iron Oxide- Selenium nanoparticles by Lyophilization, Characterization by TEM and optimization of the nanoparticles to study their effects in Cancer Theranostics-Determination of cytotoxicity of the nanoparticles on Osteosarcoma cells by PCR, MTS assays

Hyderabad Area, India

-Conducted experiments to analyze release of Nano Vitamin D3 from Bi-layer tablets of Calcium Carbonate and Nano Vitamin D3 into aqueous fluids-Involved in the formulation of the tablets with different excipients and analysis using HPLC

Master Of Science - Ms, Pharmaceutical Sciences

Courses: Concepts in Pharmaceutical Sciences- Overview of Receptors and Drug metabolism; Human physiology- Cardiac physiology.

Bachelor'S Degree, Pharmacy

Activities and Societies: -Headed teams in presenting Posters on topics such as "Probiotics" and "Nanotechnology in Pharmacy" -Secured.