• Binary vector cloning(GATE WAY Cloning)• Genomic DNA Isolation• Southern Blotting• RT-PCR• Northern Blotting• RACE(phytase gene pullout)• Phage infection• Phage lifting• Phage cDNA Library Screening through PCR• Sending/follow-up for Sequencing and Annotation• Responsible for optimistic execute project • Project development discussion with Principal Investigator• Stage development Report to Principal Investigator• Coordination, technical negotiation Team Members• Project Assistance to M.Sc. Project involved in different techniques

• Vector cloning • Eukaryotic Gene Transfer (Gene gun method) Hands on experience.• Primer Designing• Colony PCR • Transformation (E.Coli) • Restriction Digestion• Ligation• Sending/follow-up for Sequencing and Annotation• Gene Elution• Plasmid Extraction