Sayan Gupta Email & Phone Number

United States

Berkeley, California

• Develop micro-second radiolytic labeling and LC-MS approach for protein structure characterization
• LC-MS based structural studies on ion channels, metal transporter, and receptors
• LC-MS based structural characterization of novel protein therapeutic targets and epitope mapping
• Design microfluidic flow set-up for protein sample handling, ultra-fast mixing, high flow rate delivery and collection
• Development of LC-MS based structural characterization of nanoparticle-protein interactions
• Experimental designing/consultation, experimental run, and data analysis/interpretation preparing - protocols/presentations / research-proposals / manuscripts for academics and industry user for analytical and.

Center For Proteomics And Bioinformatics, Department Of Nutrition

• Experimental designing/consultation, experimental run, and data analysis/interpretation preparing - protocols/presentations / research-proposals / manuscripts for academics and industry user for analytical and.

• Development of LC-MS based protein structural characterization methods to study photosynthetic systems and bacterial microcompartments

http://www.neoproteomics.net/

Cleveland, OH & Upton, NY

• Established mass spectrometry based approach to study structure and dynamics of proteins and protein ligand interactions
• Developed novel mass spectrometry based methodology to study bound waters in protein and their dynamics
• Established methods to determine structure of nucleic acid-protein interaction and receptor-ligand binding in live cells
• Developed ultrafast radiolysis method for oxidative covalent labeling of protein and designing ultra-fast time resolved studies
• Provided graduate course lectures on Current Proteomics / Structural Proteomics
• Organized workshop, 'Solution Studies of Macromolecules: Global and Local Structure Factors' as a part of the NSLS-CFN Joint User Meeting May, 18-20, 2009 at Brookhaven National Laboratory

Upton, NY

• Built/upgraded synchrotron x-ray radiolysis and mass spectrometry facility to study protein structure and dynamics
• Built/upgraded time resolved radiolytic labeling set-up & design instruments required for the handling of proteins and live cell sample
• Designed user facility to support synchrotron x-ray footprinting for the new 3rd generation synchrotron light source - NSLS II
• Lead facility at the NSLS, BNL consisting of > 30 funded research institutions and industries
• Research support, collaboration, training and consultations to investigators (~ 40 PIs, ~ 60 academic and industry researchers)
• Reviewed research proposals and work plans as a beamlime reviewer and beamline advisory team member

Bronx, NY

• Studied protein-protein and protein-viral DNA interaction by oxidative covalent labeling and mass spectrometry
• Studied steady state and time resolved RNA folding by x-ray hydroxyl radical footprinting

• Optimized soluble/membrane protein purification from bovine heart and bacteria
• Designed cloning, site directed mutagenesis (PCR), over expression, purification and storage
• Designed protein characterization and ligand binding by bimolecular spectroscopy and activity assays
• Thermodynamics of protein folding-unfolding, protein stability, enzymatic activity

Doctor Of Philosophy (Ph.D.), Biophysics & Biochemistry

• Optimized Membrane protein purification from animal tissue and bacteria • Designed cloning, site directed mutagenesis, over expression.

Master Of Science (M.Sc.), Biophysics And Molecular Biology