Shamini Ayyadhury

Shamini Ayyadhury Email and Phone Number

Scientific Associate @ Princess Margaret Cancer Centre
Toronto, ON, CA
Shamini Ayyadhury's Location
Toronto, Ontario, Canada, Canada
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About Shamini Ayyadhury

Since Sep.2020, I have been a computational postdoctoral fellow at the University of Toronto and the University Health Network. In collaboration with the SickKids, I am currently carrying out research on adult Glioblastoma applying both experimental and computational methodologies using patient-derived samples. By applying computer vision with AI, my current work will utilize multi-dimensional imaging methods to profile patient-derived tumor tissues, to understand their extrinsic behaviour through cell/tissue morphometry and to link with intrinsic profiles obtained from single cell RNA sequencing methods. Prior to this, my graduate studies at The Neuro, McGill University, involved extensive experimental work using in-vivo models with osmotic pumps for tumor injection and drug delivery, multi-dimensional tissue imaging and analysis, single cell protein analysis(CyTOF, FACs) and molecular biological techniques such as CRISPR, single cell RNA sequencing method development and pre-clinical drug screening using once again multiple patient-derived GBM samples. Along with these research duties, I was a strong advocate for student education and training and helped train graduate students and postdoctoral fellows. My highly independent nature and skills are attributed to the 10 years of research experience prior to me coming to Canada. I was a research technician/officer in Singapore participating in Alzheimer’s disease, Regenerative medicine, Aging immunity and GBM at the Nanyang Technological University, Singapore, National Neuroscience Institute, Singapore and The Singapore Institute of Clinical Sciences, A-Star, Singapore. In between, I undertook a Masters at Imperial College, London where my graduate thesis work was on post-mortem analysis of Parkinson’s disease. These myriad experiences added value to my experience and developing my skills in molecular biological techniques (PCR, Western Blot, Elisa), high-dimensional flow cytometry, tissue culture, fluorescence imaging, immunohistochemistry. Most importantly however as a lab technician, I learnt strong managerial skills which included keeping methodological profiles of experimental records, technical methods and sample bio banking. I have helped train students and postdocs. I learnt that a well managed and organized lab is the driving force behind good research and learnt to appreciate the back-end support that labs receive. All of these experiences have made me into the scientist I am today and these are principles that guide me as I embark on my journey to realize my dream of transforming GBM research.

Shamini Ayyadhury's Current Company Details
Princess Margaret Cancer Centre

Princess Margaret Cancer Centre

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Scientific Associate
Toronto, ON, CA
Employees:
1112
Shamini Ayyadhury Work Experience Details
  • Princess Margaret Cancer Centre
    Scientific Associate
    Princess Margaret Cancer Centre
    Toronto, On, Ca
  • Princess Margaret Cancer Centre
    Postdoctoral Fellow (Visiting)
    Princess Margaret Cancer Centre
    Toronto, On, Ca
  • Panoramics - A Vision
    Director Of 'Panoramics - A Vision'
    Panoramics - A Vision Apr 2023 - Present
    Panoramics offers a vision for a pan-Canadian collaborative working cluster for spatial and single cell multi-omics technologies & projects. Panoramics promotes dialogues that will inspire, challenge and enlighten the scientist in you. Here, open dialogues are encouraged, where groundbreaking positive and negative data are shared and discussed. Panoramics encourages tutorials that will challenge you to think beyond your comfort zone. Through these dialogues and interactions, we aim to educate and encourage collaborations that will push the boundaries of the spatial and single cell world.​Together, we can re-imagine the way conversations between scientists take place, and build a panorama that goes beyond our collective imaginations.
  • Donnelly Centre For Cellular And Biomolecular Research, University Of Toronto
    Postdoctoral Fellow
    Donnelly Centre For Cellular And Biomolecular Research, University Of Toronto Sep 2020 - Present
    Toronto, Ontario, Canada
  • Princess Margaret Cancer Centre
    Postdoctoral Fellow (Visiting)
    Princess Margaret Cancer Centre Sep 2020 - Present
    Toronto, Ontario, Canada
  • Ifleed Institute Of Math And Science Llc
    Creative & Technology Director
    Ifleed Institute Of Math And Science Llc Jan 2019 - Jun 2021
    As creative director, I was part of designing and creating marketing and promotional materials as well as establishing novel steam-orientated educational materials for children.
  • The Neuro (Montreal Neurological Institute-Hospital)
    Postdoctoral Researcher
    The Neuro (Montreal Neurological Institute-Hospital) Sep 2014 - Jul 2020
    Montreal, Quebec, Canada
    My research included the identification of hierarchical model in GBM and the pre-clinical therapeutic evaluation of anti-sense compounds using in-vivo GBM models. During the course of my research, I introduced, optimized and implemented techniques for pre-clinical drug evaluation (intracerebroventricular injection, osmotic pump mediated drug delivery, optimization of precision injections in niche brain regions and dose-response evaluation), CRISPR-mediated gene editing, multiplex-immunolabelling of patient-derived and xenograft tissues, single-cell mass cytometry and single cell RNA sequencing. Furthermore, I introduced and implemented computational analysis of images and mass cytometry. My other responsibilities included training junior scientists and technicians on these techniques.
  • Singapore Immunology Network
    Research Officer
    Singapore Immunology Network Jan 2012 - Oct 2012
    Singapore
    I was part of the lab management team and provided extensive lab support through the collation and management of SLAS (Singapore Longitudinal Aging Study) samples. This involved PBMC isolation from fresh blood collected daily from elderly recruits and the subsequent multi-parametric (16-color) flow cytometry analysis in understanding T-cell diversity and changes with age. In addition, I was responsible for the management of blood samples from asthma patients obtained from the ICU and for profiling the whole blood using flow cytometry.
  • Amega Global Llc
    Research Assistant
    Amega Global Llc Jan 2010 - Jun 2011
    Singapore
    The primary objectives of my role were to maintain professional standards of product development by acting as a liason between the Product and Research Development and the Marketing departments. My responsibilities involved international travel to meet with researchers and potential manufacturing investors from the USA, Russia, Malaysia, Sri Lanka and Germany and conducting training sessions for Business Associates on product knowledge and working with doctors, researchers and service providers to continuously improvise existing products and services.
  • Singapore Clinical Research Institute
    Research Assistant
    Singapore Clinical Research Institute May 2008 - May 2009
    Singapore
    As a research officer, I worked on a series of drug-treatment assays in collaboration with Eli Lilly. The aim of this collaboration was to compare the different EC50 values of various drugs within and between cell-lines and to compare the differential efficacies of these drugs between CD133+ and CD133-cells. CD133+ cells have been postulated to hold a sub-population of rare cancer stem cells (CSCs) which have been implicated in conferring resistance to cancer cells via their capacity to survive and in expanding a cellular hierarchy of cancer cells. I worked on additional projects which included evaluating the effects of a “histone-modifier inhibitor” on cancer stem cells and in conducting drug-dosage response studies to screen for potential cancer stem cell death-inducing drug.
  • National Neuroscience Institute
    Research Fellow
    National Neuroscience Institute May 2007 - Apr 2008
    Singapore, Singapore
  • Nanyang Technological University
    Research
    Nanyang Technological University Jul 2005 - Jul 2006
    Singapore
    I was in charge of establishing a stable cell line using TNF knock-out fibroblasts from mice and murine neuronal stem cells and to use this cell-line, once established, to induce receptor activation via an agonist antibody and for subsequent intracellular signaling studies. The aim was to establish a tripletransfected cell system [APP/mPs1/mPS2] using a PC12 tet-on cell line to derive an inducible cell death system for potential anti-apoptotic studies as potential therapeutic targets for AD. Furthermore, I was also responsible for lab management and administrative duties.

Shamini Ayyadhury Education Details

Frequently Asked Questions about Shamini Ayyadhury

What company does Shamini Ayyadhury work for?

Shamini Ayyadhury works for Princess Margaret Cancer Centre

What is Shamini Ayyadhury's role at the current company?

Shamini Ayyadhury's current role is Scientific Associate.

What is Shamini Ayyadhury's email address?

Shamini Ayyadhury's email address is sh****@****onto.ca

What schools did Shamini Ayyadhury attend?

Shamini Ayyadhury attended Mcgill University, Imperial College London, National University Of Singapore.

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