I am a structural biologist and protein chemist with 20 years experience in cloning, purifying and analyzing a range of proteins using advanced Nuclear Magnetic Resonance techniques. Below is a list of my focus areas and skills. * Structural Biology of Protein-Protein and Protein-Ligand interactions.* Nuclear Magnetic Resonance (NMR) Spectroscopy applications and Methods Development. * Bacterial Pathogenesis (Iron acquisition).General Skills: Extensive protein cloning, expression and purification experience. FPLC/HPLC (Akta, BioRad, Agilent systems). Protein fusions/tags, point mutations, gene assembly. Biophysical characterization, Protein-Protein and Protein-Ligand interactions (FRET, Blitz, CD, MALDI mass spec, Multiangle Laser Light Scattering and NMR).Enzyme Kinetics: Phosphatase and pyrophosphatase assays including the development of solutions that circumvent problems in PPi detection with commonly used kits. Sortase peptide hydrolysis assays. Nuclear Magnetic Resonance: Extensive experience with protein NMR including structure determination, protein dynamics, advanced isotope labeling techniques, protein-protein and protein-small molecule interactions (CSP, PRE) and methods development in non-uniform sampling (NUS) techniques.Bacillus subtilis genetics: Gene knockouts, replacement, heterologous protein expression.