Scott Slattery Email & Phone Number

Research Triangle Park, North Carolina, Us

Durham, North Carolina, Us

In the Cell-Based Assay Division at ScitoVation, I worked toward the development, validation, and application of in vitro toxicity testing methods, and also contributed to investigations of chemical and pharmaceutical mechanisms of action. I was responsible for writing and budgeting study proposals for clients, designing and executing laboratory experiments, managing and mentoring junior scientists, evaluating and statistically analyzing experimental results, tracking project progression, managing costs, writing project reports, writing and publishing peer-reviewed papers, and managing the laboratory.

Durham, North Carolina, Us

Durham, North Carolina, Us

Durham, North Carolina, Us

Research Triangle Park, Durham, Nc, Us

Healthy cells produce reactive oxygen species (ROSs) through normal physiological processes. However, an elevated level of ROSs produces an oxidizing cellular environment, which can be highly damaging to proteins, lipids, and nucleic acids. Therefore, ROSs are combated by cellular antioxidant defense systems, which maintain a reduced cellular environment. Some xenobiotics can perturb this redox balance through various mechanisms, leading to dangerously high levels of ROSs, oxidative stress, cellular damage, and disease. I developed cell-based assays for monitoring oxidative stress in human epidermal keratinocytes after challenge with xenobiotics. In one approach, we monitored the status of the glutathione antioxidant system in an immortalized human epidermal keratinocyte cell line using a stably transduced, fluorescent, genetically encoded redox biosensor. The assay is performed kinetically and in live cells. This kinetic data allows the discrimination between temporary stress that induces an adaptive response from the cell, from more chronic stress that overwhelms the cell's ability to adapt and leads to adverse effects.

Chapel Hill, Nc, Us

Cells are morphologically dynamic, and many are highly motile. Such morphodynamics is required for normal physiology, and can also be hijacked in pathologies such as metastatic cancers. The actin cytoskeleton provides the forces underlying most cellular morphodynamics, and the Rho GTPases are central molecular regulators of the actin cytoskeleton. Understanding how cell motility and morphology are regulated by Rho GTPases is challenging because Rho GTPases are themselves regulated in a highly dynamic spatiotemporal manner. To better understand the relationship between Rho GTPase activity and actin cytoskeletal morphology, I developed novel fluorescent biosensor technologies to allow the direct visualization and quantitation of Rho GTPase activity in living, moving cells, with very high spatiotemporal resolution. Furthermore, I developed semi-automated assay methods for screening and validating new biosensor technologies to expedite future biosensor production.

Houston, Texas, Us

During this short-term postdoctoral experience in the laboratory of Michael Mancini in the Department of Molecular and Cellular Biology, I acted as Manager and Assay Development Specialist of the Integrated Microscopy Core facility, in addition to doing research on automated microscopy-based cell cycle analysis and steroid receptor co-activator function. As Manager and Assay Development Specialist, I worked with multiple researchers to develop microscopy-based approaches to their diverse research challenges, including high content analysis approaches.

Houston, Texas, Us

As a graduate student working on a Ph.D. in Molecular and Cellular Biology in the laboratory of Bill Brinkley, I studied the regulation of mitosis and cell division by Aurora family kinases. My work demonstrated that Aurora Kinase C can regulate mitotic progression. In the process of my project, I developed a cell cycle progression assay that uses a novel method for the automated classification of cells in high content microscopy images into cell cycle phases and mitotic stages.

Ph.D., Molecular & Cellular Biology

B.S., Molecular And Cellular Biology