Temporary position to support operational qualification and validation service. Upgrading qualification guide of air handling unit (AHU) and GxP cleanrooms of val-de-reuil plant. IQ, OQ of GxP equipments. software Geode, Gibraltar, pReference, ...

Ensuring successful cGMP manufacturing of CAR T-cell products as part of Phase I/II clinical trials established. Plan, manage and coordinate activities related to the buildout of the production site (oversight of qualification of cGMP suites, OQ, PQ, OOS, CAPA, Method Validation ... ) Class A aseptic manufacturing facilities of human GMO primary T cells in respect of European Pharmacopoeia rules.main achievement: first patient inclusion and injection of allogenic T cells genetically modified to control GvHD.

quality and risk assesment of human injectable medicines in ampoule packagingGlass vials/Ampoule check using automated detection of particules and defects by high resolution CMOS area cameras in confocal tandem configuration combined with high voltage leak detection.Team work with respect of cGMPs, productivity and quality continuous improvement average productivity 18-24 000 vials/hour

manufacture of hydrogel bioreactors for 3D cellular cultures for applications -in preclinical drug screening assays for pharmaceutic companies-in agrobusiness industries to produce high-value enzymatic compounds or meat substitutes-in biodefense

Like my previous position, providing services in Grant and R&D projects management with 30 additional collaborators.follow projets (15) from multiple fields (structural chemistry, drug screening, cancer, Alzheimer, cell biology, ...)improve technical efficiency of the laboratory (40% of cost reduction), write reports (national and international), follow budget, prospect for additional funding (13 000 000 euros collected in 2011) ...Field of research: providing new innovative anti-tumoral drugs based on humanized antibodies neutralizing Dependence Receptors signalling.checking of GLP respect (pre-­clinical toxicological PK/PD proof of concept studies)

follow projets (10) achieved by 30 people, improve technical efficiency of the laboratory, write reports (national and international), follow budget, prospect for additional funding (915 000 euros collected in 2010) ...Field of research: providing new innovative anti-tumoral drugs based on humanized antibodies neutralizing Dependence Receptors signalling.Beside interest in cancer therapy these drugs will become key compounds to provide a safety development of regenerative medicine based on stem cells use.Academic and Private partnership with start-up Netris Pharma

Developping industrial application in regenerative medicine of a patented biomaterial made of natural sugar polymer macroscopic physical hydrogels.proof of concept development of the use of chitosan as suitable matrix for production of vascular substitutes giving rise to a new 3rd biodegradable generation.this work leads to gain knowledge in Intellectual Property by deposit of PCT Patent 2011/151603

Pharmacogenomics of RXRs homo and heterodimers nuclear receptors. Developping ChIP on chip to decipher complex RXR/RAR nuclear receptor transcription factor networks at a genome-­wide level.Acquisition of reporting skills at the European level, managing report, negotiating deliverables, discussing/modifying strategic plans according to new technology development presentation at meeting report, working in a consortium at the international level, working in a multicultural environment.Acquisition of new technological design and production of lentivirus containing shRNA, producing and describing new antibodies, analysis of microarrays data, troubleshooting ChIP on chip technology, linear amplification of ChIP samples to perform hybridization, generating cell line from primary culture, genotyping, using CRE recombinase technology to generate KO cell lines of Nuclear Receptor coactivators.

Characterization of HP1 functions by siRNA in murine erythropoiesis using in vitro differentiation of triple knock-out mES cells (CCE cell line).Acquisition of new technological skills in confocal microscopy (FISH) and living microscopy and siRNA as well as epigenetic.Management of a £50 000/year budget within a small team (4 people), transfer of knowledge to the British lab in ES cell culture and erythroid cell differentiation

Recipient of French Hematology Society postdoctoral grant fellowship