Responsible for recruiting dentists into implant training programs and necessary tools and equipment to add implants to their practice. Built pipeline through strategic sales tactics, cold calling, trade shows, and referral generation

·Northwest Territory Representative for 15 lines of scientific equipment; responsible for keeping abreast of industry trends and legislation in order to act as a trusted advisor to customers to maximize revenue/minimize costs for future workflow planning.·Led the company conversion from paper-based to computerized CRM system that effectively tracked leads and sales cycles, reducing man-hours by 60% and leading to increased sales of 25%.·Executed consultative sales strategies, averaging 100-200 contacts per week through multiple sales techniques for the generation of new accounts, as well as maintaining an existing customer base that generated over $100,000 in revenue.·Successfully translated scientific processes into insights that led to strategic business wins, including a highly successful targeted campaign that secured an emerging pharmacy market and the re-engagement of a key customer account.

Research Technician 2, Seattle Children’s Dec 2011-June 2013900 Stewart Ave. Seattle, WA 98106 206-884-7800Original member of the Center for Pediatric Cancer Research, later named the Ben Towne Institute for Pediatric Cancer: Responsible for the production and screening of Chimeric Antigen Receptors(CARs) for use in adoptive immunotherapy treatment of hematologic and solid tumor malignanciesProjects:- Development of a CAR panel of various intracellular and extra cellular spacer domains to allow for high throughput screening of various scFv receptors- Development of a Lenti virus production for generation of lenti virus used for transduction of CARs into T-cells- Supported post-doctoral researchers and medical fellows in transduction, rapid expansion, isolation and efficacy screening of CARsExperience:- Generation of plasmids using PCR, restriction enzyme digest and ligation, and one step isothermal assembly(Gibson assembly)- Generation and maintenance of database for CARs using vector nTi plasmid construction program- Screening and sorting of CAR expressing T-cells using multi parameter flow cytometry- Isolation of Peripheral Blood Mononuclear Cells (PBMCs) with Ficoll separation- Culture and maintenance of primary T-cells and tumor cell lines as well as culture and maintenance of 293T lenti producer line- Responsible for generation of lab protocols necessary for GLP compliance as well as maintenance of electronic lab notebook according to GLP standards

Certifications: PADI Open Water Scuba Instructor, NAUI Scuba Instructor, TDI Tech 3 diver,- Helped grow company and expand product lines- Maintained rental equipment to industry standards- Coordinated, instructed, supervised and certified groups of up to twelve persons

Projects: -Development of a 200+ component internal standard for mass spectrometry analysis-Characterization of the hydrocephalus proteome by mass spectrometry-Shotgun proteomic experiment searching for biomarkers of Kawasaki’s diseaseProteomic Experience:-Develop high throughput analysis for core facility at institute-Routine maintenance on Mass spectrometer (LTQ Velos and LCQ Deca)-Automation of protocols and procedures with JANUS Liquid handler-Method development of Mass spec to decrease sample variability-Post run analysis on SPIRE (in house program) and Seaquest-Precipitation and purification of proteins -Trypsin digests of proteins -2d gel separation of protein mixtures for isolation pre-purification

Supported platform of the company through examination of viral proteins and their cellular targets to develop specific therapeutics for autoimmune and inflammatory diseases. Synthesized and cloned constructs. Cultured bacteria, cloned and prepared DNA preps for various viral factors.Grew adherent, suspension, primary, and immortalized mammalian cell lines(293T, HepG, CHO, Cos7, Hela, liver carcinoma) using aseptic sterile techniqueGene synthesized constructs for potential therapeutics via PCR. Examined expression of constructs Through transient transfection (Lipofectamine and Fugene 6) examined expression via western, silver stain, and coomassie gels.Determined functionality of constructs using dual luciferase reporter assaySet up and analyzed multi-color flow cytometry experiments using custom Q dot conjugatesOptimized immunoprecipitation (IP) pulldowns to increase protein yieldUsed AKTA Prime plus for HPLC purification of target proteinsPresented findings to peers in lab meetingMaintained laboratory notebook according to industry standards