CLINICAL TRIALS/ VIRAL PATHOGENESISDesigned and implemented a plan to purify rhesus IgA from blood requiring separation and collection of serum and conjugating an anti-IgA antibody to a column. Conducted antibody purification utilizing a GE AKTA Purifier FPLC system. Spearheaded quality control tests on purified antibodies, including gel filtration, SDS-PAGE, and non-reducing gels. Managed flow cytometry via direct and indirect stains on whole rhesus blood and isolated rhesus PBMC's. Ensured cell culture maintenance on CHO and HEK-GP293 cell lines. Subcloned transduced CHO cells by leveraging a semi solid state technique. Performed ELISA on subcloned cells. Transiently transfected GP2-293 cells. Transformed competent cells and purified plasmid from cells. Detected mycoplasma contamination by implementing PCR on cell supernatants. Evaluated endotoxin levels of purified monoclonal antibodies utilized for in vivo studies. Assessed protein concentration by conducting BCA assays. Formulated and optimized a protocol for conjugating antibodies to cyanogen bromide activated resin.Initiated a laboratory for conjugation of FITC, Biotin, APC, and PE dyes to monoclonal antibodies.Played a key role in establishing a retrovirus system to express antibodies.Drove optimization and execution of a Fab2 fragmentation protocol using pepsin digest and ion exchange chromatography.

Delivered contributions to articles appearing in several peer-reviewed publications. Managed quantitative and qualitative analysis. Proved critical to development of a PO1 grant proposal. Handled administrative duties, including ordering reagents and lab materials. Scrutinized and presented data using new software programs. Maintained laboratory equipment and performed troubleshooting. Conducted pharmacokinetic assays on potential inhibitors of amyloid beta peptides. Spearheaded Western Blot analysis on amyloid beta.Uncovered and presented new scientific data to co-workers and supervisors. Fostered working relationships through ethical, accountable, and conscientious performance.Garnered a 15% salary increase based on exemplary performance.Assured diligent quality control on purified peptides mainly via SDS-

Developed problem-solving skills by trouble-shooting several different projectsOptimized and executed a protocol for purifying the amyloid beta peptideDeveloped my listening skills by heeding the instructions of my supervisors and successfully following those instructions