Sean Lauber Email & Phone Number

Remembryo is an IVF science news site that shares summaries of the latest IVF research. I created the site in 2018 and am active on social media as "Embryoman."

Searching for news stories of interest for healthcare-related trade associations and writing summaries to be included in their weekly newsletters.

Assisting with social media content creation and writing/editing of scientific publications relating to IVF.

Remote

Worked as a freelance biology content creator preparing lessons for high school students to cover different biology topics (molecular biology, metabolism, biochemistry, genetics, physiology, etc)► Prepared dozens of written lessons (~3000 words)► Developed hundreds of question videos (~2-5 minutes)► Reviewed team member's work to ensure scientific accuracy► Followed lesson plans and Nagwa style guidelines to ensure consistency in delivery► Graphic creation for figures (Canva)

Albany, New York Area

Worked at a busy IVF clinic as an embryologist performing various tasks:► Egg retrievals, trimming, stripping of oocytes► ICSI► Culturing of embryos► Monitoring/documentation of fertilization and development using eIVF software, grading► Quality control/maintenance of instrumentation► Media preparation► Preparing embryos for transfer► Cryopreservation and thawing of oocytes and embryos► Performing trophectoderm biopsy► Handling patient communication, coordinating embryo transfers to outside facilities

Vancouver, Canada Area

Tested a number of STEMCELL Technologies (ISO 13485 certified) products to ensure their conformance to quality standards as a part of the Embryonic Stem cell (ES) team. ► Cultured embryonic and induced stem cell lines with finished products to test their quality► Performed assays to screen and select raw materials for finished products► Performed experiments to troubleshoot customer complaints or to improve QC testing procedures► Results were reported and submitted following SOPs as required by the quality management system (QMS). ► Non-conformance was documented using OOSs, NCRs, CAPA.► Created SOPs for the technology transfer of new products from RND to QC.► Member of Master Cell Bank team (cultured hybridomas to create working cell banks for manufacturing to produce antibodies)► Member of the Safety Team ► Member of the Cryo (liquid N2 monitoring) team► Chemical representative for the ES team

Followed up on experiments that related to my M.Sc. thesis involving the promotion of lung cancer by inflammation:► B16 and LLC cancer cells were used in tail vein injection models for lung cancer.► LLC cells were transduced with luciferase and followed in vivo using IVIS digital imaging.► ELISA, qPCR, and flow cytometry were performed to study the tumor infiltrating cell types. At this time I published results in the International Journal of Cancer (https://pubmed.ncbi.nlm.nih.gov/24976180/).Other tasks included:► Handled ordering of laboratory supplies and prepared reagents for day to day use. ► Mentored numerous students in understanding experimental design and technical aspects.► Developed SOPs for lab procedures with emphasis on safe handling and proper disposal.► Maintained animal colonies (genotyping, breeding, weaning)► Participated in weekly technician meetings to discuss safety/administrative issues.

Hamilton, On

Worked on addressing the effect of inflammation on lung cancer promotion:► KRAS-based transgenic mouse models for lung cancer were established► Mice were intubated with adenovirus expressing inflammatory cytokines► Tumor burden was evaluated using CT scanning (AMIRA) and histology (with ImageJ)► Inflammatory cell types were examined using microscopy and IHC► ELISAs were performed to test for increases in chemotactic markers in mouse lungWorked on various side-projects and tasks:► ELISA development with an industry collaborator► Macrophage responses to Th1/Th2 stimulation by qPCR► CT scan analysis of mouse models for lung fibrosis ► Genotyped a cell line for a mutation by DNA sequencing► Maintained mouse colonies (breeding, weaning, genotyping)► Wrote AUPs (animal utilization protocols) for work related to animal studies

Kitchener, On

Conducted 4 separate tutorial sessions for 15-20 first-year Human Biochemistry undergraduates and explained key concepts relating to biochemistry, molecular biology, metabolism and physiology. Group activities were conducted and student performance evaluated.

Pincourt, Quebec

Montreal, Qc

Investigated the mechanism of alternative protein trafficking in the yeast K. lactis by examining the differential use of transcriptional start sites as a B.Sc. thesis project which led to a paid position by the end of 2009. This involved the isolation of yeast RNA followed by reverse transcription and PCR amplification of target transcripts. These were cloned into plasmids and then transformed into bacteria before positive colonies were picked. Plasmid DNA was purified and submitted for sequencing. This led to the identification of multiple transcripts for the target gene (facilitated using BLAST). Differential translation start sites (transcription was investigated during my thesis) were then investigated. A novel knockout strain of K. lactis was generated (by recombination), and this strain was transformed with multiple expression plasmids containing the target gene lacking individual translational start sites. Mutations and restriction sites were introduced by PCR, which allowed for the ligation of multiple fragments into a series of expression plasmids for this study.

M.Sc., Medical Science

B.Sc., Cell & Molecular Biology

Dec, Natural Sciences

High School Diploma