Sean Caruthers Email & Phone Number

East Haven, CT, US

Columbus, OH, US

Stamford, Connecticut, US

In a challenging and dynamic environment, I worked across functional areas to ensure on time delivery of clinical WES/WTS testing reports. Originally designed workflows were unable to handle the volume and complications of testing and I utilized my experience and problem-solving skills to drive completion of testing and meet TAT expectations.As workflow.

Columbus, Ohio, US

• Lead variant scientist for clinical NGS panels [solid tumor and hematological malignancy – 50 and 500+ gene panels] including somatic and germline analysis.
• Pipeline developer of in house developed 50-100 gene myeloid/lymphoid panel (Ion S5 system), 50 gene Thunderbolts Myeloid panel (MiSeq) and Ampliseq Hotspot 50 gene solid tumor – setting of parameters and coordination.
• Guide pipeline development of SNV/indel calling, MSI status, tumor mutation burden for in house developed comprehensive (500+ gene) hematological and solid tumor panels (NextSeq with GATK pipeline, MSINGS).
• Data analysis, organization and delivery for clinical trials of BTK inhibitors.
• Train lab team members in variant analysis of solid tumor and lymphoid (plus ibrutinib resistance) panels.
• Provide demonstrations, education and one on one sessions for NGS data analysis and reporting for residents and genetic counselors in training.

Secaucus, NJ, US

• Molecular Oncology: May 2013-
• Lead operator and analyst for the next generation sequencing 30 gene LeukoVantage™, Myeloid Neoplasm Mutation Panel based on Illumina MiSeq platform and 7 gene MPN/MDS panel based on Ion Torrent platform
• Analyst for Sanger and Pyro sequencing and Quantitative, Real Time-PCR assays from solid tumor and hematopoietic tissues
• Performance of oncology assays including BCR-ABL1 Gene Rearrangement and PML-RARA t(15;17), Quantitative PCR, Thyroid Cancer Mutation Panel (BRAF, RAS, RET/PTC, PAX8/PPAR), Colorectal Cancer Mutation Panel (KRAS.

Key Responsibilities: Ensuring all GLP requirements are met throughout the lab. Creating and following established practices and procedures for generating, recording and analyzing electronic and hard copy data. Monitoring daily operations of the lab and providing guidance and direction to subordinates. Logistical planning for all lab phases of client.

• Key Responsibilities:
• Laboratory duties: Target preparation of RNA/DNA samples for hybridization on Affymetrix GeneChips including cDNA synthesis, in vitro transcription, column and bead cleanup of nucleic acids, and quality control by.
• Target Prep Laboratory Leader: Writing/revising SOP’s and coordination of updating to Ocimum’s Biotracker LIMS from our current LIMS. Completion of GLP study workbooks and reports. Perform scheduling and training for.
• Implemented use of the Gene Chip Array Station for automated post PCR processing of SNP genotyping experiments, eliminating source of technician errors and increasing capacity over 100%.
• Updated whole transcript expression profiling process to the Ambion WT Expression protocol, more than doubling capacity and decreasing target prep time by 25%.
• Development of DNA QC assay and associated metrics.

Key Responsibilities: Responsible for all aspects of onsite manufacturing process: configuration of customer orders, prioritization of materials use, synthesis (ABI 3900 & 394, Expedite, Oligopilot, MerMade, proprietary high throughput synthesizers), purifications (HPLC, PAGE), quality assurance (OD260, capillary electrophoresis, mass spectrometry).

Athens, GA, US

Performed research in multiple plant genetics laboratories covering population genetics, transposon research, bioinformatics, gene isolation and determination of function of developmentally regulated genes.

Rockville, MD, US

Performed non clinical genetic toxicology testing using Salmonella/Mammalian Microsome Mutagenicity Assays and Unscheduled DNA Synthesis Assays.

Master Of Science (Ms), Biotechnology - Bioinformatics Concentration

Bachelor Of Science (B.S.), Biology, General