Seddon Thomas Email & Phone Number

Durham, NC, US

Raleigh-Durham, North Carolina Area

• Rebranded and analyzed existing 45-color spectral panel data to present >30 webinars in 2021 to gain insights into panel design in conventional and spectral flow cytometry
• Trained multi-site flow cytometry team and currently leading team of 4 biologists to enable launch of >200 new NovaFluor-labeled antibodies
• Supported transition of 228 existing NovaFluor-conjugated antibodies from Phitonex website to ThermoFisher.com
• Designed and executed flow cytometry experiments to support marketing and scientist-to-scientist programs including development of new >30 color backbone panel utilizing all Thermo Fisher products

Durham, North Carolina, United States

• Designed and executed flow cytometry experiments with newly developed fluorescent antibody labels utilizing both conventional and spectral flow cytometry including pioneering testing of 40- and 45-color spectral panels
• Involved with strategic planning of novel fluorescent products as a member of both business development and research teams
• Supervised and trained biological applications team in flow cytometric panel design and analysis
• Communicated with scientists to assist in experimental design and development of cutting-edge techniques across multiple platforms (flow cytometry and next gen-sequencing)

RTP, NC

• Interrogated epigenetic inflammatory memory responses in lung epithelial cells and alveolar macrophages through early-life allergen exposure in mouse models of asthma
• Studied therapeutic implications of role of Th17 cells in TLR- vs protease-mediated mouse models of asthma
• Supervised and trained new postdoctoral fellow in mouse models of asthma, handling of neonatal mice, flow cytometric analysis, cell sorting and ATAC-seq and RNA-seq isolation
• Published 8 papers including first author Mucosal Immunology paper as corresponding author and second author Journal of Clinical Investigation and Endocrinology papers
• Co-taught NIEHS-wide Research Mentor Training Course including leadership skills and ethical lab management
• Served as peer reviewer for 15 papers submitted for publication in scientific journals including Frontiers in Immunology, Journal of Allergy and Clinical Immunology (JACI), and Journal of Immunology

RTP, NC

• Isolated lung epithelial cells, dendritic cells, and alveolar macrophage from WT and MyD88 KO mice following allergen sensitization and examined gene regulation using Nanostring, Whole Transcriptome Array, RNA-seq and.
• Collaborated and trained with lung physiology laboratory (Mike Sanderson-U Mass Medical) to bring lung precision cute lung slice (PCLS) technique to NIEHS for PCLS studies of lung physiology, immunofluorescence and.
• Supervised and trained summer student in 10 week independent project, resulting in poster presentation
• Worked with bioinformatics core to analyze Nanostring, Whole Transcriptome Array, and ATAC-seq data as well as to apply SeqGL analysis to generate hypotheses about transcriptional regulation of dendritic cell biology
• Published 2 papers (including second author Environmental Health Perspectives paper) and initiated numerous studies leading to 9 additional publications as Research Fellow

Chicago, IL

• Investigated NKT cell trafficking in parabiotic mice and determined that NKT cells remain primarily tissue resident (in contrast to conventional T cells) in an integrin-mediated process
• Designed recombineering strategies to generate knock-in mice using Bacterial Artificial Chromosomes (BACs) through collaboration with Boone lab at University of Chicago
• Submitted Howard Hughes mini-proposal to add 561 nm laser to LSR II to visualize mCherry labeled T cells
• Wrote four protocols for Institutional Animal Care and Use Committee (IACUC) approval of murine studies at University of Chicago
• Published 3 papers including first and second author Journal of Experimental Medicine papers

Boston, MA

• Studied T and NKT cell recruitment to the asthmatic airways following human segmental allergen challenge
• Phenotyped tetramer-specific cytotoxic CD8 T lymphocyte (CTL) trafficking in HIV-1 infected human subjects by examining intracellular cytokines, proliferation markers, chemokine receptor expression
• Trained new postdoctoral and medical fellows in multi-color flow cytometry panel design, gating, and fluorescence compensation
• Published 9 papers including 1 first author Journal of Immunology, 1 first author New England Journal of Medicine, and 2 first author review articles

Westerville, OH

• Trained with MIT lab in synthesis of cadmium selenide Quantum Dots (Qdots) with specific fluorescent emissions spectra under inert gas (oxygen-free) conditions
• Developed initial Qdot-protein conjugation technology acquired by Invitrogen (now Life Technologies)
• Wrote 60 page technical manual for chemical synthesis of Qdots of various spectral wavelengths

Argonne, IL

• Synthesized and purified anions using inorganic chemistry techniques including inert gas glovebox
• Proposed and electrocystallized new anionic targets with donor molecule to create new organic superconductors
• Coordinated with research groups at Argonne to test newly generated organic superconductors, resulting in 3 publications

Phd, Medical Sciences In Immunology & Virology

• Analyzed the role of chemokines in CD1d-restricted NKT and T cell trafficking under homeostatic conditions as well as in Th1 (Toxoplasma.

Ba, Chemistry

• Graduated cum laude with Honors in Chemistry and Concentration in Biochemistry and Molecular Biology • Conducted independent research.