James Sefton Email & Phone Number

Boston, MA, US

Somerville, Massachusetts, US

- Carried out screen of RNA constructs to identify lead eRNAs- Developed and executed transfection-based assays for RNA expression in primary human hepatocytes and cultured cells.- Quantified protein expression with epitope tag assays (HiBit) - Measured cell viability using CCK8 kit.- Quantified protein expression with licor western blot and JESS - In vivo.

Somerville, Massachusetts, US

南京市玄武区, 江苏, CN

- Management of research cell lines, including documentation of cell database, mycoplasma testing,- Experience with flow cytometry (EGFR PE, HER2 BV421, and IL13Ra2 APC in lung and brain cancer lines)- Performed ELISAs to examine cytokine release of IL2 and IFNg using supernatant from killing assays- Cofounder of workplace culture commitee

• Cell culture (primary cell culture isolation, transfection, infections and silencing genes by siRNA)
• Biochemistry techniques and cell-based assays (Cloning, Protein-interactions assays, Luciferase assay, Elisa, qPCR, protein purification, and Western Blot)

Cambridge, MA, US

• Induced expression of chemokines CCL19b and CCL25b, in order to establish T cell recruitment in melanoma tumors
• Cell culture- cell line maintenance and transfection.
• Sub-cutaneous injections to zebrafish.
• Zebrafish Husbandry
• Setup fish crosses and collected embryos.

Shrewsbury, GB

Instructed various classes when regular teachers were absent, while adapting teaching method based upon the age group of students and subject covered

US

• Zebrafish Husbandry
• Setup fish crosses and collected embryos
• Treating embryos for various experiments
• Screening of embryos for genetic traits
• Collecting data for ongoing research projects
• Analyzed 4XUAS gene using GFP markers under florescence microscope

Medford, Massachusetts, US

- Facility/systems maintenance and zebrafish husbandry-Oversight of breeding, care, and accurate documentation of all mutant and wild type zebrafish lines.- Troubleshoot, schedule, supervise, and monitor all system maintenance and repair issues, in consultation with the Lab Administrator and PI.-Coordination with veterinary staff for regular health.

- Used CMMS and Excel (Generate Metrology Data Sheets, Release Work Orders, Data Entry, Reactivate / Remove units from service, Reverse Calibration Traceability, Calibration Schedule Extensions) - Performed analysis of calibration results and maintained calibration history files and documentation.- Assisted with calibrations- Ship / Received.

• Fed and cared for laboratory animals compliant with IACUC, USDA, and AAALAC regulations.
• Cleaned animal and facility support rooms
• Ensured laboratory, procedure rooms, and facility support rooms are clean, safe and properly stocked.
• Maintained log books related to facility maintenance, animal usage, inventory, and equipment.
• Maintained proper identification and accurate records of individual test animals.
• Performed animal injections and bleeds

Winchester, VA, US

• Instructed various classes when regular teachers were absent, while adapting teaching method based upon the age group of students and subject covered

• Daily zebrafish care included feeding, water-quality testing, and water exchanges
• Prepared water for use in system
• Cultured invertebrate to feed to fish
• Set up tanks for zebrafish crosses and collected embryos
• Examined different stages of the zebrafish embryos and separated by stage of development
• Performed literature searches

• Assisted students and faculty with use of resource technology
• Maintained library collections and facilities

Master'S Degree, Biology/Biological Sciences, General

Bachelor'S Degree, Mathematics And Marine Biology