-Contributed to product development cycle by providing feedback from customer interactions such as software issues and inefficiencies in the product driving product optimization and customer retention. -Collaborated cross-functionally with sales, product management, R&D, and applications teams to create user friendly customer training materials which allowed for increase in effectiveness and lessened down time. -Provided technical expertise and support for over 20 customers on Akoya’s… Show more -Contributed to product development cycle by providing feedback from customer interactions such as software issues and inefficiencies in the product driving product optimization and customer retention. -Collaborated cross-functionally with sales, product management, R&D, and applications teams to create user friendly customer training materials which allowed for increase in effectiveness and lessened down time. -Provided technical expertise and support for over 20 customers on Akoya’s spatial biology and digital pathology solutions to customers in the Southeast US territory. Show less

-Deployed commercial and pre-commercial spatial imaging methods to generate high-value application data for academic and biopharma clients to showcase capabilities of the PhenoCycler Fusion instrument. -Independently designed and performed multiplex spatial biology experiments for over 10 different applications and worked closely with data scientists to interpret and deliver data to customers. -Designed and produced customer-facing collateral to advertise Spatial Biology Services… Show more -Deployed commercial and pre-commercial spatial imaging methods to generate high-value application data for academic and biopharma clients to showcase capabilities of the PhenoCycler Fusion instrument. -Independently designed and performed multiplex spatial biology experiments for over 10 different applications and worked closely with data scientists to interpret and deliver data to customers. -Designed and produced customer-facing collateral to advertise Spatial Biology Services. -Worked on 25 fee-for-service projects per quarter with samples containing 40 plex or more, contributing to $1M in revenue quarterly and securing POs for instrument sales. -Participated in debriefing internal (Project Management and Lab Services leadership) and external stakeholders (customers), including service project updates and sales training for lab service offerings. -Trained 8 Field Applications Scientists on the PhenoCycler-Fusion staining and conjugation workflows to enable them to train customers in the field. Show less

-Designed and optimized assays for Akoya’s PhenoCycler-Fusion (CODEX) platform. -Led beta-test efforts to troubleshoot errors encountered on the PhenoCycler-Fusion (CODEX) platform for early-access customers. -Collaborated with Application Scientists and R&D leadership to develop novel applications for the CODEX platform.

-Performed laboratory management tasks including ordering consumables and maintenance of reagent inventory. -Tested, optimized, and validated over 25 antibodies for the PhenoCycler-Fusion (CODEX) workflow.

• Developed pull down method for Probody PK studies in Cyno Matrix• Analyzed protease activated CAR T cells through MacsQuant FACs analysis for proof of concept development.• Evaluated library of antibodies through Wes assay for reagent development.

Synthesis and Purification/Analytical Chemistry• Collaborate with analytical team to synthesize, purify, and characterize DNA and RNA oligonucleotides using MerMade 48 and Akta oligopilot for a variety of experiments. Immunostimulatory• Assess efficacy of different nucleic acid sequences and compare their functionality in Spherical Nucleic Acid (SNA) form, to understand their immunostimulatory properties such as TLR9 response.• Measure TLR9 activation with QUANTI-Blue Assay… Show more Synthesis and Purification/Analytical Chemistry• Collaborate with analytical team to synthesize, purify, and characterize DNA and RNA oligonucleotides using MerMade 48 and Akta oligopilot for a variety of experiments. Immunostimulatory• Assess efficacy of different nucleic acid sequences and compare their functionality in Spherical Nucleic Acid (SNA) form, to understand their immunostimulatory properties such as TLR9 response.• Measure TLR9 activation with QUANTI-Blue Assay using HEK Blue hTLR9 cell lines.Antisense Screening• Screen various antisense sequences through molecular methods such as RNA extraction and rt-PCR to knockdown target transcripts.• Performed organotypic skin culture to screen SNA efficacy. Show less

Relevant Courses:-Technology Commercialization Fundamentals-Balances and Kinetics for Bioprocessing-Topics in Professional Development-Bioprocess Engineering I-Bioprocess Engineering Lab-Bioprocess Engineering Lab II-Fluids and Mass Transfer for Bioprocessing-Managerial Challenges in Pharmaceutical, Biotech, and Medical Device Industries-Practical Biological Imaging-Regulatory Sciences in Biotechnology-Molecular Genetics-Biomedicine and… Show more Relevant Courses:-Technology Commercialization Fundamentals-Balances and Kinetics for Bioprocessing-Topics in Professional Development-Bioprocess Engineering I-Bioprocess Engineering Lab-Bioprocess Engineering Lab II-Fluids and Mass Transfer for Bioprocessing-Managerial Challenges in Pharmaceutical, Biotech, and Medical Device Industries-Practical Biological Imaging-Regulatory Sciences in Biotechnology-Molecular Genetics-Biomedicine and Culture-Bioseparations-Critical Thinking and Communication Show less

• Analyzed the role of genome organization on behavioral responses in mice by utilizing ChIP-Seq, qRT-PCR, and other molecular biology techniques. • Successfully quantified rates of transcript decay in a variety of cell types and correlated data to cancer progression and fear.• Collaborated with staff scientist to study the BLA region amygdala, responsible for stress and fear response.

• Analyzed the hiiragi gene, which encodes for a poly A polymerase that adds adenyl residues to 3 prime end of RNA by dissecting fly embryo brains.• Assessed RNA decay through ThioUracil-Tagging.• Performed Immunohistochemistry and used the confocal microscope to view the hiiragi gene.

The Cleary Lab studies the regulation of mRNA decay in the Drosophila Melanogaster nervous system through a novel technique called ThioUracil Tagging (TU-Tagging). Defective regulation of mRNA decay is seen in many neurological disorders such as Alzheimer’s and amyotrophic lateral sclerosis. By using this technique, we will be able to measure mRNA stability in cells of the Drosophila nervous system, identify mRNA targets, and find regulatory elements that coordinate regulated mRNAs. Through… Show more The Cleary Lab studies the regulation of mRNA decay in the Drosophila Melanogaster nervous system through a novel technique called ThioUracil Tagging (TU-Tagging). Defective regulation of mRNA decay is seen in many neurological disorders such as Alzheimer’s and amyotrophic lateral sclerosis. By using this technique, we will be able to measure mRNA stability in cells of the Drosophila nervous system, identify mRNA targets, and find regulatory elements that coordinate regulated mRNAs. Through this experience, I learned how to :• Measure mRNA decay through TU-Tagging• Use Nanodrop to analyze concentration of DNA/RNA for Turbo DNAse treatment• Be Proficient in Immunohistochemistry• Be Familiar with Confocal Microscopy• Fix Embryos for antibody staining• Be Proficient in use of PCR machine Show less