Appointed as a Theme leader for protein engineering on various CSIRO-funded research projects, including the development of nanoyeast-based diagnostics.As Chief Investigator-B received research grant from Australian Research Council for Discovery Project-2022-2024Successfully redesigned the nanoyeast and generated a new synthetic multifunctional bio-nanomaterial (SynBioNF) from the yeast cell wall.Developing advanced protein biosensors for direct measurement of GlycoRNA from exosomesCreating a single-step mutiplexed biopanning platform to control molecular evolution by deep-mining nanopore sequencesExploring bioconjugation strategies on Quantum Dots and Upconversion Nanoparticles.Designed Zinc Finger Proteins for selective detection of human telomere repeats and other pathogenic short repeats from human genomic DNA

Developed several advanced allosteric protein biosensing systems using different reporters, including glucose dehydrogenase for electrochemical detection

☛ Accomplished computational analysis and generation of sequence fingerprints for GFP-like and non-immunoglobulin proteins. ☛ Mentored graduate students in their project design and progress. ☛ Involved in the design, execution, and management of collaborative projects.

☛ Successfully designed and engineered scaffold proteins.☛ Gained extensive knowledge of therapeutic proteins and next-generation binding proteins.☛ Coordinated the development and validation of binders against sugar.☛ Hard-core experience in cutting-edge technologies like Design and Construction of Targeted Protein Library, Phage and Bacterial surface display system, High Throughput screen & selection by cell sorting in Flow Cytometry (FACS), Amber codon suppression based non-natural amino acid incorporation.

☛ Rationally designed metal-binding helical peptides.☛ Acquired vast knowledge on the modelling of metal-binding peptides.☛ Guided graduate students in their research work.☛ Hands-on experience in solid-phase peptide synthesis and HPLC purifications.

☛ Desinged protease-resistant peptide inhibitors☛ Co-expressed multiple recombinant proteins into Yeast expression system for metabolic engineering.☛ Achieved high-resolution x-ray structure by crystallising the cancer target protein and its inhibitor peptide complex.☛ Hands-on experience in recombinant protein expression, purification, characteriszation, and large-scale production techniques.

RedHat/Fedora Linux server setup and maintenance with Bioinformatics tools, Website and Network administration, TA/Hands on Training for M.S. students to use Bioinformatics tools.

Prediction of Protein-Protein Interactions in Deinococcus radiodurans using computational methods

Sequence Analysis in Bioinformatics & Practical