Senthil Kumar Sivagurunathan Email & Phone Number

Frederick, Md, Us

Cambridge, Cambridgeshire, Gb

Rockville, Md, Us

Focused on early and late-stage process development, as well as late-stage process development and validation of cell culture processes.Played a critical role in the design, execution, and analysis of process development.Authored and reviewed sections of regulatory filings, protocols, and technical reports.Hands-on experience with micro, bench, and intermediate scale-single use bioreactors.

Frederick, Maryland, Us

Lead scientist for the technology transfer, process/capability development, protocol design, CGMP manufacturing, cryopreservation, storage, and stability testing of chimeric antigenic receptor T cell (CAR T cell) products for adoptive immunotherapy towards First-in-Child phase I/II multi-center clinical trials.Write and review SOPs, process development, and technology transfer reports, manufacturing/product stability modules for regulatory submissions (CMC), qualification/validation studies, with a good understanding of cGMP guidelines. Work collaboratively with cross-functional teams, internal and external stakeholders to support CGMP compliance, regulatory submissions, and data dissemination.Process development and technology transfer of expression, and purification of a multiprotein complex, self-assembling virus-like nanoparticle (VLP) for pre-clinical and first-in-human clinical trials.Assay Development for the detection and quantification of VLPs at all stages of the production process (upstream/in process/downstream).Train and educate a team of scientists in CAR T manufacturing process, VLP upstream/downstream manufacturing, and quantitative analytical methods.

Boston, Ma, Us

Discover the essentialome of Malaria parasite employing state-of-the-art molecular genetic screens.Explore the role of pseudokinases in Plasmodium biology by employing advanced gene knock-down strategies.Generated ~150 constructs comprising genetically complex expression cassettes and validated the constructs by sequencing, western blot, immunofluorescence assays.Created plasmodium cell-lines stably expressing proteins with knock-down and affinity epitope tags.Employed affinity pulldown coupled to mass spectrometry to identify interaction partners of a novel protein involved in parasite organization in blood cells.Identified a novel role of a previously uncharacterized protein in DNA condensation in malaria parasites during blood stage replication.Initiated and implemented new approaches to improve lab methods and actively participated in the troubleshooting of technical problems experienced by lab personnel.

Conducted scientific and market due diligence for investors (VCs and angels) looking to fund early stage investments in the life sciences and healthcare market.Managed and advised a team of analysts conducting due diligence projects.Organized and lead teleconference meetings with clients and analysts.Reviewed reports from analysts to ensure achievement of technical milestones and deliverables.Delivered cursory diligence report for life science companies at the OneMedForum Conference.

Bloomington, Indiana, Us

Explored a key component for infection of Toxoplasma gondii (Toxoplasmosis) causing parasite.Dissected the minimal functional domains of a non-nuclear methyltransferase involved in parasite motility.Employed classic biochemical techniques to purify actin and myosin from rabbit muscles.Designed methyltransferase truncation constructs, expressed and affinity purified encoded proteins from bacterial expression systems.Employed radioactivity based enzyme assays to evaluate the activity of methyltransferase truncations.Purified protein complexes (GAP) involved in parasite motility by affinity purification techniques.Prepared presentations, manuscripts, and abstracts for publication.

Kansas City, Mo, Us

Established key methods to study the structure-function relationships of a motor protein in a fungal system. Pioneered the development of a three-phase protein purification strategy (Capture/Intermediate-purification/polishing – CIPP based) to isolate a “megadalton-sized” motor protein complex to homogeneity from the fungal system.Routinely purified milligram quantities of native (untagged) proteins from mammalian cells using large-scale biochemical techniques.Employed creative molecular genetic techniques to construct fungal strains expressing fluorescent/affinity tagged proteins of interest and strains harboring mammalian mutations of interest (a proof-of-concept design).Presented research work at several scientific meetings and conferences.Initiated and implemented new approaches to improve lab methods and actively participated in the troubleshooting of technical problems experienced by lab personnel.Maintained lab equipment including ÄKTA HPLC and ordered lab supplies.

Actively interacted with students, conducted/supervised lab sessions, graded tests and assignments

Madurai, Tamil Nadu, In

Zoology

Doctor Of Philosophy (Phd), Molecular Biology And Biochemistry

Master Of Science (Ms), Cellular & Molecular Biology

Master Of Science (M.Sc.), Gene Technology

Bachelor Of Science (B.Sc.), Zoology/Animal Biology