Sesha Raghavan Krishnamachari Email & Phone Number

Chapel Hill, NC, US

United States

Identifying new opportunities and drive customer support along with sales growth using value selling skills in all segments of the Pharmaceutical, Biopharma, clinical, academia, CRO & CDMO markets within the Midwest territory (IL, MN, WI).Conducted on-site product demonstrations and technical seminars, attend conferences and vendor shows.Providing.

Little Rock, Arkansas, United States

Developed a novel immunological assay kit for rapid neurodegenerative biomarker assay. Screened new compounds developed by in silico modeling as a potential therapeutic drug. Used CRISPR- Cas based gene-editing methods to develop mutant stable cell lines.

Department Of Laboratory Medicine And Pathology

Established and managing a new lab for developing novel quantitative histology techniques for high throughput multiplex staining. Train new lab members and students in bio-marker studies for Alzheimer’s disease by using old histology techniques, combined with modern analytical methods. Manage lab funds, assist in developing grant proposals, organize data.

Minneapolis

Involved in screening DNA adducts in Formalin fixed (FFPE) samples by Immunohistochemistry and compare to Mass spectrometry. Established a new tissue culture facility & study multiple DNA carcinogen adducts in different cell lines & animal models. Developing in vitro methods & transfer the technology for DNA retrieval & adduct analysis in human FFPE.

El Paso, Texas Area

Paul L. Foster School of Medicine, El Paso, TexasCenter of Excellence in Neuroscience Studied the normal functions of the proteins alpha Synuclein, Protein Phosphatase 2A involved in Parkinson’s disease pathology. Developed and analyzed new drugs that have considerable potential for treating synucleinopathies such as Parkinson’s disease. Identified novel.

Center For Biotechnology & Genomics, Lubbock, Texas Area

Assisted the Proteomics Core Facility with equipment set up and tested the samples on mass spectrometer. Standardized HPLC, LC-MS/MS protocols to identify proteins and peptides using gel free proteomics approach

Collaborative Project of Texas Tech University – Center for Biotechnology & Genomics, with the Garrison Institute on Aging – Texas Tech University Health Sciences CenterProfiled human cerebrospinal fluid proteins in Alzheimer’s disease using proteomics. Analyzed protein up/down regulation in patient samples using Mass Spectrometer (MALDI TOF/TOF), HPLC, 2D.

Taught graduate students for a laboratory course titled “Cloning, overexpression and characterization of 3-alpha hydroxysteroid dehydrogenase/ carbonyl reductase from Comamonas testosteronii”. Optimized project protocols and co- authored a Laboratory Manual used as a reference book for this course

Awarded 'Best Employee of the Month' - Nov '09 & Feb '10 for sincerity at work and excellence in customer relationship.

Monitored fermentation operations & system parameters, assisted R&D department in sample testing and quality control

Trained on upstream and downstream techniques of fermentation process.Worked for quality management team under the R&D department. Secured knowledge about formulation techniques and marketing methods of fermented product.

Ms, Biotechnology

B.Tech, Biotechnology

High School, Science