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Experienced, hands-on scientist/engineer interested in opportunities that push science and technology to the next level and positively impact human health. Education: Ph.D. Molecular Biology and B.S. Electrical Engineering. Specializing in nucleic acid chemistry, biophysics, molecular biology, electrochemistry, and microfluidic chip design. Recent experience with surface-based isothermal DNA amplification, modified nucleotides for electronic DNA sequencing, DNA polymerase engineering, and single molecule detection. Past experience with RNA structure probing, protein—RNA, and small molecule—RNA interactions.SPECIFIC SKILLS:Molecular Biology & Next-Gen Sequencing: Sequencing by synthesis chemistries and detection methods, NGS library preparation, nucleic acid sample prep, DNA amplification (PCR and isothermal), nucleic acid structure and biophysics, modified nucleotides and oligonucleotides, RNA structure probing, and numerous molecular cloning and biochemistry methods (too many to list). Familiarity with Unix command line and Python programming.Engineering: Microfluidic chip design, fabrication, and testing. 3D CAD with Solidworks, multiphysics FEA with COMSOL, circuit simulation with SPICE, LabView for breadboard control. Familiarity with silicon, polymer, and elastomer microfabrication technologies. Familiarity with impedance spectroscopy, and other electrochemical methods. Past experience with MOSFET device physics and CMOS circuit design.
Axiome Bio
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Principal ScientistAxiome BioMenlo Park, Ca, Us
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CtoNexgen Biotools Jun 2023 - PresentNexgen Biotools is solving the library prep bottleneck in nextgen sequencing workflows with novel microfluidics solutions.
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Director Of Emerging TechnologyUltima Genomics Apr 2022 - May 2023Fremont, California, UsResponsible for developing the next iteration of Ultima's clonal amplification technology. -
Vice President, Core Technology R&DRoswell Biotechnologies Sep 2021 - Mar 2022San Diego, Ca, UsWorked mainly on adapting Roswell's single-molecule detection technology for DNA sequencing applications. -
Principle Scientist, Research FellowGenapsys, Inc. Mar 2015 - Sep 2021Since successful completion of proof-of-concept for the microfluidic library preparation project, I have been conducting R&D projects aimed at improving GenapSys core DNA sequencing technology. This work has involved development of improved sequencing methods and chemistry that are compatible with GenapSys CMOS chips.
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ConsultantGenapsys, Inc. Feb 2014 - Feb 2015I have been responsible for the development of innovative microfluidic chips and supporting instrumentation to implement NGS library preparation. This has involved creation of a novel digital pneumatic architecture and low cost manufacturing methods to produce devices and systems that are a suitable match for GenapSys’ compact, low-cost DNA sequencing instrument. The digital pneumatic architecture is a programmable engine compatible with multi-step processing, multiple component mixing, magnetic bead-based purification, high-temperature incubation, and PCR.
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CofounderAthena Biosystems Jan 2013 - Jan 2014Athena is exploiting the latest developments in robotics to produce a new liquid-handling laboratory robot that provides radically increased flexibility and programming ease at an affordable price. Athena's technology provides access to virtually the full range of standard molecular biology tools and methods including: microliter-level liquid-handling, evaporation control, PCR, magnetic bead processing, centrifugation, vortex mixing, and a facile interface to microfluidic chips. Unlike competing systems, Athena's robot will be the first compact, low-cost liquid-handling robot to completely automate NGS library preparation protocols.
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Principal ScientistGenapsys Aug 2012 - Nov 2012I worked on their novel and innovative DNA sequencing technology based on electronic detection of polymerase activity. I was mainly occupied with characterization and debugging of the sensor chip using a microfluidic breadboard system and electronic instruments for measuring small currents.
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Senior Staff & Principal ScientistIntegenx Oct 2008 - Aug 2012IntegenX has been developing microfluidic chip technology based on pneumatically actuated on-chip pumps and valves constructed with PDMS membranes. I worked on a number of successful and interesting projects there that involved both my molecular biology and engineering skills, beginning with development of a microfluidic chip for mRNA amplification in collaboration with Samsung. More recently, I designed microfluidic chips for parallelized (24X) Illumina NGS library preparation (gDNA and mRNA), PCR, and single cell capture. These efforts included development of a novel parallelized, digital pneumatic microfluidic architecture and improved chip fabrication materials and methods that eliminated problems associated with the permeability of PDMS. In addition, I was technical lead for a DITRA-funded novel pathogen detection project based on DNA amplification (MDA with phi29 polymerase) and Illumina NGS, as well as for the research phase of a project to develop an NGS library preparation liquid-handling robot, which is now an IntegenX product.
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Senior ScientistArcxis Biotechnologies 2006 - Oct 2008UsI was mainly involved in two activities: first, invention of methods and devices for automated, miniaturized mRNA amplification, which involved successful application of for a Phase I SBIR grant to the National Institute of General Medical Sciences (NIGMS), and second, development of methods and chemistries for automated and miniaturized total RNA, polyA+ RNA, and genomic DNA sample preparation. These methods produced nucleic acids with yields and purities equaling or exceeding those of market leading kits. mRNA generated with polyA+ mRNA purification chemistry was successfully amplified and analyzed on Affymetrix microarrays. -
Staff ScientistApplied Biosystems 2004 - 2006Waltham, Ma, UsI was involved with invention and development of miniaturized, sample-to-answer nucleic acid processing systems comprising polymeric, laminated microfluidic chips for nucleic acid purification with nanoporous aluminum oxide membranes, and thermocycling for RT-PCR. These chips interfaced with LabVIEW-driven valves, syringe pumps, and epi-fluorescence optics. In addition I was involved in the development of an automated silica-based pathogen DNA and RNA purification system using a polymeric, laminated microfluidic chip carrying a silica membrane, and LabVIEW-driven valves and syringe pumps. -
CofounderChemautomaton 2002 - 2004ChemAutomaton proposed development of technologies for automated, massively parallel, target-directed small molecule (ligand) discovery and optimization, based on iterated DNA-programmed organic synthesis, target-specific selection, and PCR amplification. Such methods represent an alternative to traditional medicinal chemistry and high-throughput screening drug discovery technologies. They are essentially elaborations on SELEX (or in-vitro genetic selection) methodology developed for aptamer discovery, in which highly diverse synthetic DNA libraries are used not only to encode but also program synthesis of small molecules, which remain associated with their encoding DNA oligomer (thereby linking genotype and phenotype). Specifically, ChemAutomaton proposed to miniaturize and automate a procedure developed at Stanford University, using microfluidic technology to produce a bench-top small molecule (ligand) discovery/optimization instrument. Business plans and SBIR grant applications were prepared, and the technology was presented to potential investors.
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Principal ScientistCaliper 2000 - 2003Glendale, California, UsCaliper was developing a microfluidic chip for real-time PCR based on a novel thermocycling strategy that involved joule heating of PCR buffer in microfluidic channels. I was first able to successfully conduct proof-of-concept experiments, and I then designed a microfluidic "sipper chip" that could detect SNPs in gDNA. I subsequently developed methods for conducting alternating electric current directly into microfluidic channels based on exploitation of the electrical double layer present at metal-electrolyte interfaces. -
ConsultantIndependent Consultant Jun 1998 - Jun 1999For IBIS Therapeutics: Identification of potential antimicrobial drug targets in ribosomal RNA and design of “RNA analogs" useful in high-throughput screening. I identified sub-domains in bacterial 16S and 23S ribosomal RNA with potential utility as targets for small molecule protein synthesis inhibitors and designed small RNA analogs for use in in-vitro screens.For Molecular Machines: Invention of methods for in-vitro evolution of nucleic acids and small molecules. I generated IP for these systems.For Allelix (Toronto, Canada): Invention of array-based, high-throughput RNA structure probing for discovery of small molecule RNA-binders. I adapted methods developed at UC Santa Cruz for RNA structure probing (during my PhD research) for drug discovery applications.
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Assistant ProfessorUmass Medical Center 1992 - 1998My postdoctoral research at Cold Spring Harbor Laboratory was concerned with the human homeodomain- (actually POU domain-) containing transcription factor Oct-1. As an assistant professor, I successfully competed for and competed a NIH R01 research grant for research on Oct-1—VP16 interactions. These studies involved the development of a yeast two-hybrid method to screen for human analogs of viral transcriptional activator proteins. In addition, I successfully competed for and competed a second NIH R01 research grant for research on RNA structure and function, and my group published original research in the leading scientific journals Nature and Cell on this subject. We identified small molecule binders of the decoding region of 16S rRNA and the HIV Rev-Response Element (RRE) using chemical structure probing methods.
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Postdoctoral FellowCold Spring Harbor Laboratory 1988 - 1992Cold Spring Harbor, New York, UsSuccessfully competed for and competed an NIH postdoctoral training grant. I was able to show that the viral transcription factor VP16 recognizes amino acids in the homeodomain (DNA-binding domain) of the human homeodomain protein Oct-1, an unexpected and significant observation at the time that resulted in several publications including the leading scientific journal Nature. -
Phd ResearcherUc Santa Cruz 1984 - 1988Santa Cruz, Ca, UsMy PhD research in Professor Harry Noller's lab at UCSC concerned protein--RNA interactions in the 30S subunit of the E. coli ribosome. I participated in the development of new chemical/enzymatic structure probing and footprinting methods that relied on reverse transcription of modified RNA. Using these methods we were able to localize binding sites for numerous 30S subunit proteins, as well as protein-induced conformational changes, in 16S rRNA. We then used the known three-dimensional coordinates for the centers of mass of the proteins to speculatively fold 16S rRNA in three-dimensions, based on these binding sites. -
Device PhysicistIntel Corporation 1980 - 1984Santa Clara, California, UsI was very fortunate to assist in the development of Intel's CMOS process technology (P444). I was involved with: validation of design rules for new device fabrication technology, characterization of the performance of CMOS static RAM test vehicle, full-die simulation (using Intel’s proprietary SPICE derivative) of a 64K CMOS dynamic RAM, characterization of 64K CMOS dynamic RAM performance, and circuit and layout modifications for revisions of 64K CMOS dynamic RAM.
Seth Stern Skills
Seth Stern Education Details
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University Of California, Santa CruzMolecular Biology Of Rna -
Uc IrvineElectrical Engineering
Frequently Asked Questions about Seth Stern
What company does Seth Stern work for?
Seth Stern works for Axiome Bio
What is Seth Stern's role at the current company?
Seth Stern's current role is Principal Scientist.
What is Seth Stern's email address?
Seth Stern's email address is se****@****sys.com
What is Seth Stern's direct phone number?
Seth Stern's direct phone number is +165033*****
What schools did Seth Stern attend?
Seth Stern attended University Of California, Santa Cruz, Uc Irvine.
What are some of Seth Stern's interests?
Seth Stern has interest in Thermoplastic Elastomers (Tpes), In Vitro Evolution, Isfets, In Vitro Genetic Selection, Ngs Library Prep, Small Molecule Rna Interactions, Rna Structure And Function, Chemfets, Carbon Nanotube Fets, Epigenetics.
What skills is Seth Stern known for?
Seth Stern has skills like Genomics, Molecular Biology, Pcr, Biotechnology, Dna, R&d, Microfluidics, Life Sciences, Lifesciences, Microarray, Rna Isolation, Labview.
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