Shaji Abraham, Ph.D. Email & Phone Number

A highly flexible and driven biomedical scientist [Ph.D.] with 15+ years of research experience in genomics, molecular and cellular Biology, oncology, and platelet biology. I have built my career on driving translational research and advancing drug discovery.Encompass expertise in designing and executing preclinical studies, leveraging cutting-edge molecular techniques like CRISPR, lentiviral vectors, transcriptomics, and protein biochemistry to uncover therapeutic targets and biomarkers. Additionally skilled in antibody screening and target validation, functional assays, and assay development for antibody therapeutics. My research includes antibody therapeutics, small molecule inhibitors, and gene therapies for cancer and thrombotic diseases. Possess excellent qualitative and quantitative skills, outstanding leadership qualities, and adeptness at advancing research with significant outcomes.Key Achievements• 25+ peer-reviewed publications• 16+ national/international presentations• 2 patents and a federally funded grantAreas of Expertise• Antibody therapeutics, Oncology and thrombosis research• Biomarker discovery, antibody and target validation• Cell-based and in vivo assay development• Translational and functional genomicsI am passionate about harnessing innovation to discover and develop therapies that address unmet medical needs. Let’s connect!📧 shajiabraham2024.JB@gmail.comResearch Experiences:Oncology: Prostate and Bladder cancer research. Molecular Oncology, Biomarker Identification, and Validation by genomic and proteomic approaches. Anti-tumor drug targets and mechanism of action studies.Hemostasis and thrombosis: Megakaryocytes and platelet biology.Cell Culture and assay development: Designing and execution of in-vitro assays, cell-based assays, cell-proliferation, reporter assays, maintaining cell cultures, transfection, transduction, stable cell lines.Functional Genomics: Transcriptome profiling-mRNAs and miRNAs, gain/Loss of function studies.Protein Biochemistry: Protein Isolation, Quantification, Separation, Purification, identifying Protein-Protein Interaction, identification of protein by Immunostaining/ Immunofluorescence methods.Cloning: Cloning gene of interest in vectors, CRISPR/CAS9 knock-out, over-expression of proteins.Molecular techniques: qPCR, RT-PCR, genotyping, sequencing, screening cDNA libraries, immunoblots, immunoprecipitation, DNA-RNA blots.Cell-based Model systems: Gene overexpression/ Knockdown of proteins by transient or stable methods for designing model system for Preclinical Research.

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  Sr. Research Scientist

  Immunome, Inc.

  Apr 2023 - Present - Exton, Pennsylvania, United States

  • Led high-throughput screening of patient-derived monoclonal antibodies using FACS and confocal microscopy to identify lead candidates for cancer therapeutics.• Designed and optimized antibody validation assays, driving advancements in therapeutic development.