Shalaka Samant, Ph.D. Email and Phone Number
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• Experienced industrial research leader with a proven record in conceptualizing and writing successful grant proposals.• Seasoned researcher with a proven record in the use of microarray-based, genome-wide approaches for identifying novel bacterial virulence genes. • Strong independent research experience as a postdoctoral fellow at the Yale School of Medicine (bacterial pathogenesis) and University of Texas-Houston (signaling in macrophage development). • Extensive experience in microbial strain engineering to construct production strains for various industrial enzymes, therapeutic proteins, probiotic strain engineering and characterization and whole cell bio-transformations for green chemistry applications.• Extensive experience in writing / reviewing international journal articles. Specialties: Bacterial transposon mutagenesis, whole genome screening techniques involving the use of microarrays and bacterial virulence gene identification, aseptic techniques for bacterial cultures, construction and characterization of targeted knockouts in Gram-negative and Gram-positive bacteria, microarray-based transcriptional profiling, DNA and RNA isolation, cloning, northern blot, PCR, quantitative real-time PCR, sequencing, recombinant protein expression and purification,SDS-PAGE.
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Founding ScientistKiwi Biosciences Jul 2021 - PresentCambridge , Ma, Us -
Founder And ConsultantBiombrella Aug 2019 - Present
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Scientific Consultant And WriterKolabtree Feb 2019 - May 2021London, Gb -
Manager-Ii Discovery BiologyAnthem Biosciences Private Limited Apr 2018 - Feb 2019Bangalore, Karnataka, In• Strain engineering and evolution of various probiotic strains (designer probiotics)• Successfully developed production strains for production of menaquinone, phytase, lipase and S-adenosylmethionine. -
Manager-I, Discovery BiologyAnthem Biosciences Private Limited Jun 2014 - Mar 2018Bangalore, Karnataka, In• Successful applicant at the SBIRI (Deptt. of Biotechnology, Govt. of India) research funding initiative (10% success rate) where the proposals were judged for innovation, social and national relevance and industrial application (2014) for generation of a thermotolerant probiotic strain• Development of whole cell biotransformation platforms for pharmaceutically important biotransformations such as transaminations, phosphorylations and oxygenations• Development of novel expression systems for biosimilars -
Deputy Manager-Ii, Discovery BiologyAnthem Biosciences Private Limited Apr 2012 - Jun 2014Bangalore, Karnataka, In• Successful applicant at the SBIRI (Deptt. of Biotechnology, Govt. of India) research funding initiative (10% success rate) where the proposals were judged for innovation, social and national relevance and industrial application (2012) for generation of a secretory E. coli platform• Developed a novel array of bacterial expression plasmids for secretory protein expression in E. coli that has resulted in a PCT application (PCT/IB2012/051730). • Developed a PCR-based assay for quantification of various probiotic strains from a mixture following culture on selective media.• Successfully engineered a non-sporulating strain of Bacillus subtilis for large scale therapeutic protein production. -
Deputy Manager-I, Discovery ResearchAnthem Biosciences Private Limited Jan 2010 - Mar 2012Bangalore, Karnataka, In• Successful applicant at the BIRAP (Deptt. of Biotechnology, Govt. of India) research funding initiative (15% success rate) where the proposals were judged for innovation, social and national relevance and industrial application (August 2011)• Instrumental in cloning and expression of industrial enzymes and biologics in bacterial and yeast systems. Engineered bacterial strains to facilitate industrially relevant biotransformations e.g. ketoreductions.• Led projects dealing with generation and characterization of gene knockdown and over-expression models in mammalian cells for developing cell-based assays. -
Postdoctoral FellowThe University Of Texas Health Science Center At Houston Apr 2009 - Oct 2009Houston, Texas, Us• Investigated the role of MAP kinases in macrophage differentiation and proliferation using primary murine bone marrow cells. • Studied the role of adaptor molecules in signal transduction in an ovarian tumor microenvironment. • Trained in breeding of mice, purification and culture of bone marrow macrophages, production and western blot analysis of cell lysates, cell proliferation assays and kill curves. • Optimized the generation of lentivirus carrying shRNA constructs targeting JNK kinases and the transduction of murine bone marrow macrophages with these lentiviruses. -
Postdoctoral Research AssociateYale University School Of Medicine Apr 2008 - Apr 2009New Haven, Ct, Us• Investigated novel virulence genes in the pathogen Campylobacter jejuni using genome-wide screening of transposon insertion libraries• Designed and developed a novel microarray-based approach for the identification of genes that are important for the survival of Campylobacter jejuni in human intestinal epithelial cells. -
Graduate Research AssistantUniversity Of Illinois At Chicago Aug 2002 - Mar 2008Chicago, Il, UsInvestigated mechanisms for bacterial survival and growth in human blood. Identified two biochemical pathways that are critical for the growth of both Gram-negative and Gram-positive bacteria in human blood. Identified novel virulence determinants of the deadly pathogen Bacillus anthracis that are required for evasion of antimicrobial peptides and metabolic requirements that are critical for growth in human blood. -
Teaching AssistantUniversity Of Illinois At Chicago Dec 2002 - May 2003Chicago, Il, Us
Shalaka Samant, Ph.D. Skills
Shalaka Samant, Ph.D. Education Details
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University Of Illinois ChicagoPharmaceutical Biotechnology -
University Of MumbaiPharmacy -
Vidya Mandir, Dahisar
Frequently Asked Questions about Shalaka Samant, Ph.D.
What company does Shalaka Samant, Ph.D. work for?
Shalaka Samant, Ph.D. works for Kiwi Biosciences
What is Shalaka Samant, Ph.D.'s role at the current company?
Shalaka Samant, Ph.D.'s current role is Founding Scientist, Kiwi Biosciences | Author.
What is Shalaka Samant, Ph.D.'s email address?
Shalaka Samant, Ph.D.'s email address is sh****@****bio.com
What schools did Shalaka Samant, Ph.D. attend?
Shalaka Samant, Ph.D. attended University Of Illinois Chicago, University Of Mumbai, Vidya Mandir, Dahisar.
What skills is Shalaka Samant, Ph.D. known for?
Shalaka Samant, Ph.D. has skills like Protein Expression, Qpcr, Sds Page, Pcr, Mutagenesis, Biochemistry, Rna Isolation, Dna, Sequencing, Microarray, Cell Culture, Molecular Biology.
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