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Full stack development on custom web applications for research scientists at AbbVie Stemcentrx, allowing users to record and manage laboratory research experiments. Examples include:• Data collection, tracking, and reporting for in vivo research studies• Research task scheduling system• Status tracking and requesting system for internal deliverablesIn other words, developing a custom laboratory information management system (LIMS).Technologies used include:• Groovy/Grails• Ruby on Rails• MySQL• JavaScript

Performed DNA library preparation and sequencing on the Illumina MiSeq platform.Performed array comparative genomic hybridization on the Agilent microarray platform.Performed DNA genotyping, sequencing, and data analysis using Sanger sequencing for breast cancer genes, colorectal cancer genes, and carrier status genetic testing panels.Optimized DNA preparation by consolidating DNA isolation processes and reducing processing time by half.

Ran validation/verification testing on the HiSeq 2500 and 1500 platforms for the release of new hardware, software, and reagent kits. Collected and analyzed output data on a range of control samples and experiment parameters and confirmed if values were within specifications.Trained customer hardware support technicians on proper protocols for preparation of samples, handling of reagents, setup of software, and troubleshooting of hardware issues. Collaborated with and provided input for various departments such as marketing, software, hardware engineering, and chemistry development to deliver finalized HiSeq 2500.Tested new flow cell prototypes and compiled data for comparison with standard flow cells.

Taught SAT Math preparation courses, Algebra I, Geometry, Algebra II, Trigonometry, and Pre-Calculus.Taught English writing classes for middle and elementary students.Prepared teaching materials for the company’s use in SAT preparation.

Analyzed the gene regulatory network controlling aboral ectoderm specification in the sea urchin embryo, in order to provide a foundation for further research on individual genes.Using PCR, designed and synthesized reporter constructs with transcription factor binding sites on for the genes dlx, irxa, nk2.2, and tbx2/3. Performed DNA and RNA extractions and quantitative PCR (real-time PCR) to measure relative expression levels of reporter constructs once injected into an embryo.Used a newly designed qPCR 13-tag system that allowed for the injection of multiple constructs into each embryo, expediting efficiency of cis-regulatory analysis.

Analyzed the cis-regulatory modules governing expression of the gene foxa in Strongylocentrotus purpuratus, using genetic knockdowns and synthesis of reporter constructs containing Brachyury and Hox11/13 transcription factor binding sites.Determined the kinetics and temporal expression effects of the transcription factors Hox11/13 and Brachyury on the expression of foxa by creation of reporter constructs with portions of regulatory DNA and constructs with point mutations at putative binding sites with PCR, showing that the Brachyury transcription factor is an activator of the foxa gene, and Hox 11/13 is an early activator of foxa and controls expression kinetics and boosts expression levels.Measured relative expression levels and kinetics of synthesized constructs at various time points with qPCR, as well as DNA and RNA extraction and purification.