Shashank Ramesh Email & Phone Number

Madison, WI, US

Boston, Massachusetts, United States

• Handling and maintaining three mass spectrometry equipment with regular cleaning, QC runs, and maintenance checks.
• Executing and analyzing global proteomics mass spectrometry data for various collaborative projects.
• Quantifying reversible cysteine modifications across proteomes using Cysteine-Reactive Phosphate Tags (CPT), tandem mass tag (TMT)-multiplexing, and immobilized metal affinity chromatography (IMAC) to help in vitro and.
• Identifying proteome changes using thermal proteome profiling on collaborative projects for analyzing protein-compound interactions and protein stability.

Atlanta, Georgia, United States

• Molded gelatin cluster wells from silicon master–-mold to selectively capture CTC clusters from blood and CSF samples.
• Performed immunofluorescence-based staining to visualize and detect specific surface markers on CTCs from patient samples.
• Developed de novo multiplex RT-PCR based stratification of medulloblastoma patients using newly identified biomarkers.
• Executed the de novo method on 6 controls, 15 CSF, and 40 blood samples of medulloblastoma patients successfully.

Atlanta, Georgia, United States

• Assisted in teaching undergraduate courses in Biology and Applied Physiology, including BIOS 1107 and APPH 1050 for 1000 students.
• Conducted tutorials, led discussion sessions, and provided one-on-one assistance to students to enhance their understanding of course materials by conducting weekly reviews and office hour sessions.
• Assessed student performance through quizzes, exams, and assignments, and provided constructive feedback to enhance academic progress.
• Developed strong organizational and multitasking abilities by managing various teaching-related tasks
• Delivered 3 – 4 lectures to 150 – 170 students every semester.

West Point, Pennsylvania

• Streamlined the pronase assay workflow by developing a novel filter-assisted sample preparation and on-column clean-up, reducing sample requirement and processing time with easier handling.
• Facilitated untargeted identification of drug-related adducts through differential analysis between control and dosed samples, reducing reliance on software-based identification.
• Elucidated adduct structure with MS/MS spectra using Compound Discoverer software.
• Proposed mechanisms for the formation of untargeted drug-related adducts based on analytical findings.

Atlanta, Georgia, United States

• Worked towards understanding how multiple levels of epigenetic mechanisms are regulated in normal development and disease states
• Examined genetic variation by genotyping H1 transgenic mice models using PCR and Gel Electrophoresis.
• Performed and analyzed gene expression profiling between different benign and malignant cancer samples.
• Mentored three undergraduate students to perform molecular biology techniques, ensuring adherence to GLP and safety protocols.

Bengaluru, Karnataka, India

• Quantified N- glycans in mAb samples using UPLC to assess biotherapeutic solubility and metabolic fate.
• Characterized mAb samples using peptide mapping, multi-attribute monitoring (MAM), and disulfide mapping using LC-MS.
• Analyzed the intact mass of biotherapeutics using MALDI-TOF instruments.
• Identified the secondary and tertiary protein structures of antibody samples using circular dichroism (CD) spectrophotometry.

Master Of Science - Ms, Biology/Biological Sciences, General, 4.0/4.0

Fully Funded

Bachelor Of Engineering - Be, Biotechnology, 8.91/10