Shih-Heng Chen Email and Phone Number
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►A research scientist in the Viral Vector Core in National Institute of Environmental Health Sciences (NIEHS/NIH), with a Ph.D. in Microbiology and Immunology. Responsible for virus production, titration, and validation, including lenti/retroviruses, adeno-associated virus (AAVs), Zika virus, Rabies virus, respiratory syncytial virus (RSV) and human immunodeficiency virus (HIV). I am also developing new viral vector tools for gene delivery both in vitro and in vivo. Meanwhile, with prior eight years research experience in Neuropharmacology Group at NIEHS, I have a strong background in investigating the role of the neuroinflammation in mental disorders and neurodegenerative diseases. Specialize in design, develop, and implement experiments in neuron-glia interactions, neuroinflammation, neurodegeneration, and neuro-infectious diseases. STRENGTHS • Strong background in virology research with hand on experience in virus production, virus isolation, purification, titration and validation. • Proven records in developing innovative approaches and techniques to study virus-host interaction, neuro-immune and neuron-glia interactions, such as developed in vitro and in vivo experimental models of inflammation-mediated neurodegeneration. • Solid skills and troubleshooting ability in virus and cell cultures, immunostaining, genetic manipulation, protein biochemical analysis, and cell-based assay development.• Well-developed teamwork abilities and leadership skills and able to manage multiple projects through collaboration, such as listened to the issues and discussed possible solutions; encouraged team members after several experiments failed; assisted in increasing team productivity by contributing my skills and connect people with different expertise. • Well-demonstrated writing and oral presentation skills with more than 60 articles/reviews and poster presentations in international conferences. ► To find out more contact: shihhengc@gmail.com ☎ (919) 541-7799
National Institute Of Environmental Health Sciences (Niehs)
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Viral Vector Core BiologistNational Institute Of Environmental Health Sciences (Niehs) Jan 2017 - PresentResearch Triangle Park, Nc, Us(1) Producing and tittering of viruses, including AAV, Lenti/Retro virus, Zika virus, Rabies, Respiratory syncytial virus (RSV), human immunodeficiency virus (HIV), Sandbis virus and Adenovirus.(2) Creation of stable cell lines(3) Provision of ready-made viruses(4) Consultation in molecular cloning and plasmid construction(5) Consultation in viruses and viral vectors application(6) Training and Education for viral vector packaging
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Research FellowNational Institute Of Environmental Health Sciences (Niehs) Jun 2012 - Jan 2017Research Triangle Park, Nc, UsI have embarked on several very ambitious projects to study the interaction between microglia and astroglia in inflammation-mediated neuro-degenerative diseases using different primary culture systems, neuro-degenerative rodent models and molecular biology techniques. Despite the fact that glial interaction has been known for some time, the basic mechanism governing this regulation in neuro-degenerative stage remains unclear. The long-term goal is to translate these studies from the bench towards the treatment of neuro-degenerative disorders, including Parkinson's disease.I also collaborate with other laboratories at NIEHS to study the molecular mechanism of how microglia-derived free radicals cause neuronal damage in neuro-degenerative diseases.The following is the summary of my work at this position:• Studied immune regulation of glial cells in the central nervous system by detecting pro-inflammatory cytokine and neurotrophic factor expressions using real-time PCR, ELISA and Western Blot. • Developed and optimized protocols to detect proteins from tissues and from culture supernatant.• Created a state-of-the-art environment for tissue cultures, molecular cloning and protein work in the laboratory, including theories and technologies.• Provided technical advice and supports to solve research problems for the supervisor and other group members.• Reported high-quality and reproducible results to the supervisor on a regular basis.• Attended weekly group meeting for critical data analysis and brainstorming.• Coached and trained postdoctoral fellows and postbac interns.• Researched new methodologies and equipment for the laboratory. Obtained competing quotes and presented cost-benefit analysis to the supervisor.• Supervised the research group to comply with the safety regulations of the institute.
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Visiting FellowNational Institutes Of Health, National Institute Of Environmental Health And Sciences Jun 2010 - Jun 2012Bethesda, Md, UsI performed experiments to study the molecular mechanism of histone deacetylase (HDAC) inhibitor elicited neuroprotective effect. The HDAC inhibitor I used was suberoylanalide hydroxamic acid (SAHA). I found that SAHA could trigger neurotrophic factor (e.g. GDNF and BDNF) released from astroglial cells. SAHA could enhance histone protein hyperacetylated in GDNF promoter region. This study offered a potent therapeutic drug for neurodegenerative disease therapy. The following is the summary of my work at this position:• Studied mechanism of neurotrophic gene regulation in astroglia using Chromatin-Immunoprecipitation (ChIP) assay, Western blot, ELISA, real-time PCR and primary cell culture. • Provided technical advice and supports to solve research problems for the supervisor and other group members.• Reported high-quality and reproducible results to the supervisor on a regular basis.• Attended weekly group meeting for critical data analysis and brainstorming.• Coached and trained postdoctoral fellows and postbac interns.• Researched new methodologies and equipment for the laboratory. Obtained competing quotes and presented cost-benefit analysis to the supervisor.• Supervised the research group to comply with the safety regulations of the institute. -
Post-Doctoral FellowNational Cheng Kung University Jun 2008 - Jun 2010Tainan, Taiwan, TwIn Professor Ru-Band Lu's lab, Institute of Behavioral Medicine, College of Medicine -
Graduate Research AssistantNational Cheng Kung University 2002 - 2007Tainan, Taiwan, Tw• Studied the role of cellular transcription factor early growth response 1 (Egr-1) in herpes simplex virus type 1 (HSV-1) infected cells. I found that virus could use host cell transcription factor to increase viral replication by activating promoters of viral productive cycle genes through binding to its corresponding sequences in the viral promoters.• Proposed a new model of how drug-resistant herpes virus reactivation from infected neurons. • Designed experiments and developed protocols to verify the proposed model, including promoter activity assay, viral gene and protein expressions, ChIP assay, and in vivo animal studies.• Presented results in written and oral formats in seminars, and poster presentation in international conferences.
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Visiting ScholarUniversity Of Pennsylvania Nov 2005 - Dec 2005Philadelphia, Pa, UsDr. Nigel Fraser’s labI was invited to visit Dr. Fraser's lab for one month to demonstrate how to induce HSV-1 reactivation from latent-infected mouse brain. -
Visiting ScholarHarvard Medical School Jun 2004 - Jul 2004Boston, Ma, UsDr. Donald Coen’s labI stayed in Dr. Coen;s lab for one month to study Real-time PCR assay to detect HSV-1 genome in virus-infected brain tissues. -
Undergraduate InternNational Cheng Kung University 1998 - 2001Tainan, Taiwan, TwStudy the reactivation mechanisms of drug resistant herpes simplex virus• Proposed a new model of how drug-resistant herpes virus reactivates from infected neurons.• Developed a new protocol to identify wild type and drug resistant viruses in the nervous tissues.• Awarded by National Science Council Award for Undergraduate Summer Research
Shih-Heng Chen Skills
Shih-Heng Chen Education Details
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National Cheng Kung UniversityMicrobiology And Immunology -
National Cheng Kung UniversityGeneral
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崇先高中
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崇先高中
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崇先高中
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崇先高中
Frequently Asked Questions about Shih-Heng Chen
What company does Shih-Heng Chen work for?
Shih-Heng Chen works for National Institute Of Environmental Health Sciences (Niehs)
What is Shih-Heng Chen's role at the current company?
Shih-Heng Chen's current role is Biologist at National Institute of Environmental Health Sciences (NIEHS).
What is Shih-Heng Chen's email address?
Shih-Heng Chen's email address is nu****@****.com.tw
What schools did Shih-Heng Chen attend?
Shih-Heng Chen attended National Cheng Kung University, National Cheng Kung University, 崇先高中, 崇先高中, 崇先高中, 崇先高中.
What are some of Shih-Heng Chen's interests?
Shih-Heng Chen has interest in Helping Others In Need, Marathon, Home Improvement Projects, Baseball, Basketball, Photography, Macbook Pro, Music, Apple Products, Imac.
What skills is Shih-Heng Chen known for?
Shih-Heng Chen has skills like Western Blotting, Cell Culture, In Vivo, Immunohistochemistry, Molecular Biology, Flow Cytometry, Virus Culture, Plaque Assay, Real Time Pcr, Pcr Primer Design, Chromatin Immunoprecipitation, Promoter Reporter Assay.
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