Shraddha Gupta Email & Phone Number

Luxembourg, Luxembourg, Lu

New Brunswick, Nj, Us

• Optimized TEMPO -oxidation of oligosaccharides at different pHs, time points, with and without a co-oxidant and analyzed the oxidized oligosaccharides using TLC• Characterized hydrazide bond between oligosaccharides and stable beads using hydrazine – aldehyde chemistry• Performed binding studies of GFP, GFP_CBM17 & GFP_CBM3a to glycan conjugated beads• Amplified and synthesized recombinant plasmid using PCR – PIPE/SLIC mutagenesis• Transformed the recombinant plasmid into competent bacterial cells using heat shock method• Amplified and expressed recombinant supercharged proteins from large scale cell culturing of E.coli cells• Purified recombinant supercharged proteins by (Nickel) affinity chromatography using FPLC• Documented protocols for experimental procedures, prepared reports and presentations for meetings

Houston, Texas, Us

• Working on two projects requiring assay development and validation to detect cancer and tuberculosis mutations• Identifying variant specific fluorophores (F) and quenchers (Q) with thermodynamic favorability to amplifytuberculosis mutant genes along with optimization of F:Q ratios, their designs, number of PCR cycles, etc• Designing and performing various q-PCR assays followed by data analysis and results• Defining limit of detection to be 0.1% VAF (Variant allele frequency)• Defining quantitative range of detection of mutant cancerous lines (determining %VAF detected @ dCt ranges)• Identifying blockers that significantly amplify the various unknown cancerous human cell lines by q-PCR for thepurpose of pooling them into a master mix as part of a developing assay kit• Establishing robustness of blockers in amplification of target mutations• Establishing reproducibility of data by q-PCR over multiple mixtures of different mutant and normal cell lines

• Instruct a class of 24 first year undergraduate students, twice a week• Organize and conduct 12 experiments: Paper chromatography, Net ionic equations, Density of matter, Reactivity of metals, Volumetric analysis: An acid base titration, water of hydration, Enthalpy of formation etc• Conduct one-on-one discussions with students and graded assignments fairly

• Assisted a student who is handicapped to perform the lab experiments: Reactivity of metals, Net ionic equations, Determination of Molar volume of a compound, etc• Studied the experiments before each lab and listened carefully to the instruction given at the start of the sessions• Strictly adhered to the lab rules and operations

In

Project: Extraction of silica from rice husk• Conducted experiments to extract silica gel from rice husk collected from a local rice factory• Generated activated carbon from the waste obtained from the extraction experiments to reduce the carbon footprint of the process• Carried out mass balance, energy balance and cost analysis to understand the feasibility of a hypothetical industrial plant, thus establishing an eco-friendly viable process to generate silica from waste

Master’S Degree, Chemical And Biochemical Engineering

Bachelor Of Engineering - Be, Chemical Engineering