Bioassay Development for Mab's and Insulins Method Development and Method qualification of invitro functional cell based assays Antibody dependent cell cytotoxicity etc.) for assessment of bio similarity of biological products like Monoclonal Antibodies for Breast cancer, and various autoimmune disorders.  Peripheral Blood Mononuclear Cell (PBMC’s) isolation from whole blood using Ficoll paque technique.  ADCC assay with frozen PBMC’s, transformed jurkat cells, NK cells. Insulin Analogues :Receptor phosphorylation assays: Method Development and Method Qualification for various insulin analogues using Alpha screen platform. Sample analysis for all the developed methods.Mitogenic and Metabolic Assay development and qualification Statistical Data Analysis/Biosimilarity studies Non-clinical statistical analysis to demonstrate bio-similarity at the invitro bioassay level using Parallel Line analysis (PLA software) and SoftmaxPro GxP software.